Vδ2 T细胞在2型糖尿病中的功能及临床研究

The function and clinical study of Vδ2 T cell in patients with type 2 diabetes mellitus

目的探讨Vδ2 T细胞在2型糖尿病中的功能并与临床指标进行相关性分析。方法收集2018年6月至2019年6月在该院诊治的40例2型糖尿病患者(2型糖尿病组)和35例体检健康者(健康对照组),采用流式细胞术检测2型糖尿病组和健康对照组Vδ2 T细胞的比例;采用CD4磁珠阴性分选法和流式细胞术分选法分选2型糖尿病组和健康对照组Vδ2 T细胞,体外刺激后采用酶联免疫吸附试验(ELISA)检测细胞因子白细胞介素(IL)-17和干扰素-γ(IFN-γ)水平;统计分析Vδ2 T细胞的比例与空腹血糖、糖化血红蛋白和空腹C肽的相关性。结果2型糖尿病组空腹血糖和糖化血红蛋白水平高于健康对照组,提示血糖控制不佳。流式细胞术结果显示,Vδ2 T细胞的数量在2型糖尿病组中降低;ELISA结果显示,在2型糖尿病组Vδ2 T细胞释放的IL-17和IFN-γ减少。相关性分析发现,Vδ2 T细胞的比例与空腹血糖和糖化血红蛋白水平呈负相关(r=-0.411,-0.324;P<0.05),与空腹C肽无相关性(P>0.05)。结论在2型糖尿病中Vδ2 T细胞的数量和细胞因子释放的功能均降低,且Vδ2 T细胞的数量与空腹血糖和糖化血红蛋白水平呈负相关,提示Vδ2 T细胞可能与糖尿病患者血糖控制情况存在相关性。

Objective To explore the function of Vδ2 T cells in patients with type 2 diabetes mellitus and to analyze the correlation between Vδ2 T cells and clinical indexes.Methods According to the criteria of diagnosis and elimination of type 2 diabetes mellitus,40 cases of type 2 diabetes mellitus and 35 healthy persons from June 2018 to June 2019 in the hospital were collected in the study.Flow cytometry was used to detect the proportion of Vδ2 T cells in type 2 diabetes mellitus group and healthy control group.Vδ2 T cells were selected from patients with type 2 diabetes mellitus group and healthy control group by CD4 magnetic beads negative sorting and flow cytometry.The levels of interleukin(IL)-17 and interferon-γ(IFN-γ)were detected by ELISA after stimulation in vitro.The correlation between the proportion of Vδ2 T cells and fasting blood glucose,glycosylated hemoglobin and fasting C-peptide was analyzed statistically.Results The levels of fasting blood glucose and glycosylated hemoglobin in type 2 diabetic group were higher than those in healthy control group.Flow cytometry results showed that the number of Vδ2 T cells decreased in type 2 diabetes mellitus group.The results of ELISA showed that IL-17 and IFN-γreleased by Vδ2 T cells decreased in type 2 diabetes mellitus group.Correlation analysis showed that the proportion of Vδ2 T cells was negatively correlated with fasting blood glucose and glycosylated hemoglobin(r=-0.411,-0.324;P<0.05),but was not correlated with fasting C-peptide(P>0.05).Conclusion The number of Vδ2 T cells and its cytokine secretion ability are decreased in patients with type 2 diabetes mellitus,and the number of Vδ2 T cells are negatively correlated with fasting blood glucose and glycosylated hemoglobin,suggesting that Vδ2 T cells may be correlated with the control of glucose in patients with type 2 diabetes mellitus.