Cd24a和前列腺素代谢相关酶在PCOS小鼠模型卵巢颗粒细胞中表达水平的实验研究

Expression Levels of Cd24a and Prostaglandin Metabolism Related Enzymes in Ovarian Granulosa Cells of PCOS Mice

目的:探讨Cd24a分子和前列腺素代谢相关酶在多囊卵巢综合征(polycystic ovary syndrome,PCOS)小鼠模型卵巢颗粒细胞中的表达水平。方法:取20只雌性C57BL/6小鼠,随机分为对照组(正常小鼠)和实验组(应用脱氢表雄酮建立PCOS小鼠模型),每组各10只。对照组小鼠于颈背部连续皮下注射20天芝麻油溶液(0.1 m L/100 g)。实验组应用脱氢表雄酮(6 mg/100 g)联合芝麻油溶液(0.1 m L/100 g)连续颈背部皮下注射20天。经苏木精-伊红染色观察两组小鼠卵巢组织病理学改变。应用实时荧光定量PCR法对小鼠卵巢颗粒细胞中Cd24a分子和前列腺素代谢相关酶m RNA表达量进行检测。结果:实验组体重显著高于对照组(P<0.05);实验组小鼠卵巢呈多囊样改变,卵泡中颗粒细胞数量减少,闭锁卵泡增多,闭锁卵泡直径明显大于对照组;实验组Cd24a分子和前列腺素代谢相关酶m RNA表达量较对照组存在显著差异(P<0.05)。结论:PCOS小鼠卵巢颗粒细胞中Cd24a分子和前列腺素代谢相关酶表达量异常,提示Cd24a分子可能与PCOS疾病发生相关。

Objective: The purpose of this research is to study the expression levels of Cd24 a molecules and prostaglandin metabolism related enzymes in ovarian granular cells of polycystic ovary syndrome(polycystic ovary syndrome, PCOS) mouse mode.Methods: Twenty female C57 BL/6 mice were randomly divided into the control group(normal mice) and the experimental group(dehydroepiandrosterone was used to establish PCOS mouse model), with 10 mice in each group. Control group was given sesame oil solution(0.1 m L/100 g). In the experimental group, dehydroepiandrosterone(6 mg/100 g) combined with sesame oil solution(0.1 m L/100 g) was injected subcutaneously in the neck for 20 days. Ovarian histopathological changes were observed by hematoxylineosin staining. m RNA expression levels of Cd24 a molecule and prostaglandin metabolism related enzymes in mouse ovarian granule cells were detected by real-time fluorescent quantitative PCR. Results: The body weight of the experimental group was significantly higher than that of the control group(P<0.05). The ovary of the experimental group showed polycystic changes, the number of granulosa cells decreased, the number of atresia follicles increased, and the diameter of atresia follicles was significantly larger than that of the control group. m RNA expression levels of Cd24 a molecule and prostaglandin metabolism related enzymes in the experimental group were significantly different than those in the control group(P<0.05). Conclusion: Abnormal expression of Cd24 a molecule and prostaglandin metabolism related enzymes in ovarian granulosa cells of PCOS mice suggested that Cd24 a molecule was associated with PCOS disease.