一种改良的大鼠颈静脉自主给药插管及其应用

Improved intubation of rat jugular veins for self-administering drugs

目的介绍一种改良的大鼠颈静脉插管制作方法和颈静脉插管手术方法,并应用于一项大鼠自主给药实验研究中,评估该插管设计的使用寿命和应用效果。方法对29只已完成食物训练的雄性SD大鼠用改良的插管进行颈静脉插管手术,进行丙泊酚和可卡因的自主给药模型建立,分析术后5个月的动物淘汰情况。结果丙泊酚自主给药模型和可卡因自主给药模型均建立成功,术后动物淘汰原因主要有濒死、堵管、漏液,术后第1~5个月,每个月与插管直接相关的淘汰率分别为7%、7%、10%、31%和11%,在月淘汰率在10%以内尚能接受的情况下,该插管组件的使用寿命预计至少3个月,部分插管使用寿命能够长达5个月甚至可能5个月以上。动物濒死的原因与肝肾严重损伤、电解质紊乱和体内炎症有关。结论改良的用于大鼠颈静脉自主给药的插管有诸多优点,本文所设计插管组件的插管大鼠最佳使用寿命可长达3个月,能够满足自主给药实验的基本需求,故应用效果良好,可推广使用。

Objective To introduce an improved method for intubating the jugular vein and for jugular vein intubation surgery in rats and to apply this method to a rat self-administration experimental study to evaluate the service life and application effect of this intubation design.Methods Twenty-nine male Sprague-Dawley rats who had completed food training were intubated with a modified cannula in the jugular vein.Self-administration models of propofol and cocaine were established,and elimination from the rats was analyzed at approximately 5 months postoperation.Results Both the propofol and cocaine self-administration models were successfully established.The main reasons for postoperational drug elimination were death of the rats,blocked tubes,and fluid leakage.In months 1-5 after surgery,the intubation-related elimination rates for each month were 7%,7%,10%,31%,and 11%,respectively.With an acceptable monthly elimination rate of<10%,the service life of the intubation is expected to be at least 3 months,and some can be as long as 5 months or more.Rat deaths were related to severe liver and kidney damage,electrolyte disturbances and inflammation.Conclusions Improved intubation of rat jugular veins for drug self-administration has many advantages.The optimal service life of this intubation technique was up to 3 months,which meets the basic requirements of self-administration experiments.Therefore,this application effect is good and should be promoted.