蝙蝠葛诺林碱对大鼠局灶性脑缺血的神经保护作用

Neuroprotective Effect of Daurinoline on Focal Cerebral Ischemia in Rats

目的:观察蝙蝠葛诺林碱对大鼠持续性局灶性脑缺血的影响。方法:采用大脑中动脉线栓法制备大鼠局灶性脑缺血模型,随机分为假手术组(Sham)、缺血模型组(Isch)、蝙蝠葛诺林碱(10 mg·kg^-1·d^-1与20 mg·kg^-1·d^-1)治疗组,尼莫地平组(阳性对照,0.8 mg·kg^-1)和溶剂组(阴性对照)。大鼠持续缺血24 h后观察各组的神经行为变化,检测脑梗死体积、脑组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:与模型组相比,蝙蝠葛诺林碱可以剂量依赖性改善大鼠持续性局灶性脑缺血引起的神经行为障碍,减少脑梗死体积,降低脑组织MDA水平,提高SOD活性(P<0.05);高剂量组的作用与尼莫地平作用相似。结论:蝙蝠葛诺林碱可通过增强脑组织SOD活性,提高清除氧自由基能力,从而发挥对脑缺血损伤的保护作用。

Objective:To observe the protective effect of daurinoline on focal cerebral ischemia in rats.Methods:Middle cerebral artery occlusion(MCAO)was used to set up the model of focal cerebral ischemia in rats.The rats were randomly divided into the sham operation group,ischemia model group,daurinoline treatment groups(10 mg·kg^-1·d^-1and 20 mg·kg^-1·d^-1),nimodipine group(positive control,0.8 mg·kg^-1)and solvent group(negative control).After 24 h continual ischemia,the changes of neural activity,cerebral infarct volume,MDA content in brain and SOD activity were detected in rats.Results:Compared with model group,daurinoline could ameliorate neurological deficit score and decrease infarct volume induced by MCAO in rats in a dosage-dependent manner,and it could reduce MDA content in brain and enhance SOD activity(P<0.05).The effects of daurinoline at high dosage were similar to those of nimodipine.Conclusion:The results suggest that daurinoline can play a neuroprotective effect on the injury induced by middle cerebral artery occlusion through the improvement of SOD activity and the ability enhancement of scavenging oxygen free radicals.