高效液相色谱法同时测定复合调味料中2种甜味剂和3种防腐剂

Simultaneous determination of 2 kinds of sweeteners and 3 kinds of preservatives in compound seasoning by high performance liquid chromatography

目的建立高效液相色谱法(high performance liquid chromatography,HPLC)同时测定复合调味料中安赛蜜、苯甲酸、山梨酸、脱氢乙酸和糖精钠5种食品添加剂的分析方法。方法样品经超纯水提取,取离心后的水相清液,过0.22μm滤膜,色谱柱为C18柱,流动相为甲醇-0.02 mol/L乙酸铵,柱温为30℃,流速为1.0 mL/min,紫外检测器在230 nm检测,高效液相色谱仪分析。结果本方法5种组分在0.5~50μg/mL范围内具有良好的线性关系,相关系数大于0.99995,加标回收率为91.6%~110.5%,相对标准偏差为0.1%~4.0%。检出限在0.0005~0.0018 g/kg范围,定量限在0.0015~0.0059 g/kg范围。结论该方法操作简单,结果准确,适合复合调味料中安赛蜜、苯甲酸、山梨酸、脱氢乙酸和糖精钠的同时检测。

Objective To establish an analytical method for the simultaneous determination of 5 kinds of food additives(acesulfame potassium,benzoic acid,sorbic acid,dehydroacetic acid and saccharin sodium)in compound seasoning by high performance liquid chromatography(HPLC).Methods Samples were extracted by water,and the aqueous solution after centrifugation was filtered through 0.22μm membrane.The chromatographic column was C18,mobile phase was methanol-0.02 mol/L ammonium acetate,column temperature was 30℃,and flow rate was 1.0 mL/min.The sample was detected by UV-detector at 230 nm and analyzed by HPLC.Results The concentration and response of each component showed a good linear relationship in the range of 0.5-50μg/mL with correlation higher than 0.99995.The average recoveries and relative standard deviations(RSDs)were in the range of 91.6%-110.5% and 0.1%-4.0%,respectively.The limits of detection and the limits of quantitative were in the range of 0.0005-0.0018 g/kg and 0.0015-0.0059 g/kg,respectively.Conclusion This method is simple,accurate,which is suitable for determination of 5 kinds of food additives in compound seasoning.