食用菌中硫酸链霉素残留的免疫快速检测

Rapid Immunological Detection of Streptomycin Sulfate Residues in Edible Fungi

研究基于间接竞争酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)方法,建立一种快捷、灵敏、准确的检测食用菌中硫酸链霉素残留的方法。以杏鲍菇、双孢菇、香菇等食用菌为样品原材料,采用磷酸钠缓冲液(phosphate buffered saline,PBS)提取和5%三氯乙酸净化的简单前处理方法即可基本消除基质影响。得到的校正曲线检测限为:(0.2±0.05)μg/L,灵敏度为:(0.87±0.15)μg/L;5种食用菌样品中的硫酸链霉素的添加回收率为:88.5%~99.5%,变异系数小于7.7%,其结果与高效液相色谱串联质谱法(high performance liquid chromatography-mass spect-rometry,HPLC-MS)一致。

A rapid,sensitive and accurate method for the detection of streptomycin sulfate residues in edible fungi was established based on the application of indirect competitive enzyme-linked immunosorbent assay(indirect competitive,ELISA)method.The edible fungi such as pleurotus eryngii,agaricus bisporus and lentinus edodes were used as sample raw materials,and the matrix pretreatment method can be basically eliminated by phosphate buffer sodium(PBS)extraction and 5%trichloroacetic acid purification.The obtained detection limit of the calibration curve was(0.2±0.05)μg/L,the sensitivity was(0.87±0.15)μg/L;the recovery of streptomycin sulfate in the five edible fungi samples was 88.5%-99.5%,and the coefficient of variation was less than 7.7%,the results were consistent with high performance liquid chromatography-mass spectrometry(HPLC-MS).