水稻OsRRK1蛋白的进化分析及其亚细胞定位

Evolutionary Analysis and Subcellular Location of OsRRK1 Protein in Rice

水稻(Oryza sativa L.)OsRRK1(Rop-interacting receptor-like kinase 1)蛋白,属于类胞质受体激酶RLCK VI家族成员.通过将水稻的OsRRK1蛋白序列与小米、高粱、玉米、二穗短柄草、葡萄、苜蓿、大豆、棉花和拟南芥等9个物种的同源蛋白序列进行比对,发现OsRRK1蛋白与大多数植物的同源蛋白序列相似性都很高,特别是单子叶植物,相似性高达75%以上.依据NCBI公布的水稻OsRRK1的ORF(open reading frame)序列设计引物,从水稻籼稻品种Kasalath叶片cDNA中扩增得到目的片段,构建了OsRRK1:GFP融合瞬时表达载体,转化水稻原生质体对其进行亚细胞定位.激光共聚焦观察结果表明,水稻OsRRK1蛋白定位在细胞质中.

Rice(Oryza sativa L.)OsRRK1(Rop-interacting greceptor-like kinase 1)protein,belong to a member of the cytoplasmic receptor like kinase RLCKVI family.Through amino acid sequence alignment analysis,the OsRRK1 protein was compared with the homologous protein of millet,sorghum,corn,brachypodium distachyon,grape,alfalfa,soybean,cotton and arabidopsis.It was found that the homology of OsRRK1 protein with the homologous gene in most plants was very high,especially in monocotyledonous plants,the sequenced similarity was more than 75%.According to ORF sequence of rice OsRRK1 published by NCBI,primers were designed to amplify the target fragment from the cDNA of rice variety Kasalath leaves.OsRRK1:GFP fusion transient expression vector was constructed,and rice protoplasts to detect its subcellular localization was transformed.The results of laser confocal observation showed that rice OsRRK1 protein localized to the cytoplasm.