伴和不伴有桥本甲状腺炎的甲状腺乳头状癌的蛋白表达差异

Differential protein expressions in papillary thyroid carcinoma patients with or without Hashimoto′s thyroiditis

目的研究伴和不伴有桥本甲状腺炎(HT)的甲状腺乳头状癌(PTC)的蛋白表达差异。方法收集2014—2016年在中国医学科学院肿瘤医院诊治的PTC患者资料,其中伴HT患者103例,不伴HT患者109例。制备组织芯片,利用免疫组化法检测伴和不伴有HT的PTC中鼠类肉瘤滤过性病毒致癌基因同源体B1(BRAF)、血管内皮细胞生长因子(VEGF)、细胞周期蛋白D1(cyclin D1)、间皮细胞(MC)、CD56和半乳糖凝集素3(Galectin3)蛋白的表达水平,分析蛋白表达差异。结果BRAF蛋白在伴和不伴HT的PTC中的阳性表达率分别为55.4%(36/65)和63.6%(42/66),差异无统计学意义(P=0.336)。VEGF蛋白在伴和不伴HT的PTC中的阳性表达率分别为25.7%(19/74)和25.8%(17/66),差异无统计学意义(P=0.991)。cyclin D1在伴和不伴HT的PTC中的阳性表达率分别为93.4%(71/76)和97.6%(80/82),差异无统计学意义(P=0.206)。MC在伴和不伴HT的PTC中的阳性表达率分别为86.1%(62/72)和83.5%(71/85,),差异无统计学意义(P=0.654)。Galectin3在伴和不伴HT的PTC中的阳性表达率分别为98.7%(76/77)和97.5%(78/80),差异无统计学意义(P=0.583)。CD56在PTC组织和癌旁甲状腺滤泡上皮细胞中的阳性表达率分别为27.4%(32/117)和65.0%(76/117),差异有统计学意义(P=0.001);在伴和不伴HT的PTC中的阳性表达率分别为35.5%(24/68)和16.5%(13/79),差异有统计学意义(P=0.009)。结论BRAF、VEGF、cyclin D1、MC和Galectin3蛋白在伴有HT的PTC的发病机制中未发挥明显特异作用;CD56的表达情况可以作为PTC诊断的参考指标;CD56可能与伴有HT的PTC的发生有关。

Objective To explore the differential protein expressions in papillary thyroid carcinoma(PTC)with or without Hashimoto′s thyroiditis(HT).Methods Tissue microarray was prepared and the protein expression levels of v-Raf murine sarcoma viral oncogene homolog B1(BRAF),vascular endothelial growth factor(VEGF),cyclinD1,mesothelial cell(MC),CD56 and Galectin3 in the PTC tissues with or without HT were detected by immunohistochemical staining.Results The positive expression rates of BRAF protein in the PTC tissues with or without HT groups were 55.4%(36/65)and 63.6%(42/66),respectively,without significant difference(P=0.336).The positive expression rates of VEGF protein in the PTC tissues with or without HT groups were 25.7%(19/74)and 25.8%(17/66),respectively,without significant difference(P=0.991).The positive expression rates of cyclin D1 protein in the PTC tissues with or without HT groups were 93.4%(71/76)and 97.6%(80/82),without significant difference(P=0.206).The positive expression rates of MC protein in the PTC tissues with or without HT groups were 86.1%(62/72)and 83.5%(71/85),without significant difference(P=0.654).The positive expression rates of Galectin3 protein in the PTC tissues with or without HT groups were 98.7%(76/77)and 97.5%(78/80),without significant difference(P=0.583).The positive expression rates of CD56 in the PTC tissues and adjacent thyroid follicular epithelial cells were 27.4%(32/117)and 65.0%(76/117),respectively,and the difference was statistically significant(P=0.001).The positive expression rates of CD56 in PTC tissues with or without HT were 35.5%(24/68)and 16.5%(13/79),respectively,and the difference was statistically significant(P=0.009).Conclusions There are no significant differences in the expressions of BRAF,VEGF,CyclinD1,MC and Galectin3 between the PTC tissues with or without HT.However,the significantly differential expression of CD56 between the two group suggests that CD56 may be related to the pathogenesis of PTC with HT.CD56 may be used as a potential molecular marker in PTC diagnosis.