咳可灵合剂对RSV感染小鼠IL-6、IL-8水平的影响

Effect of kekeling mixture on the levels of IL-6 and IL-8 in RSV infected mice

目的:探究咳可灵合剂对呼吸道合胞病毒(RSV)感染小鼠白介素-6(IL-6)、白介素-8(IL-8)水平的影响,进而为RSA的治疗提供理论依据。方法:以60只健康的雄性小鼠为研究对象,随机分为正常组、模型组及咳可灵低、中、高剂量组和利巴韦林组,共计6个组别,每组各10只,对RSV建立完成后各组的一般情况进行观察、记录;使用免疫印迹法(WB)检测小鼠肺部组织病变情况,使用酶联免疫吸附测定法(ELISA)测定各组小鼠肺部灌洗液及肺组织中的IL-6、IL-8水平。结果:正常组一般情况明显优于RSV感染小鼠;正常组小鼠肺泡上皮细胞较为完整,支气管及肺间隔完好无损,RSV感染模型组中的小鼠肺泡上皮细胞破损,血管肿胀充血;RSV感染后小鼠肺泡灌洗液及肺组织中IL-6、IL-8水平均高于正常组,差异具有统计学意义(P<0.05);与模型组相比,经治疗后小鼠肺泡灌洗液及肺组织中IL-6、IL-8水平明显下降,差异具有统计学意义(P<0.05),各组小鼠肺泡灌洗液及肺组织中IL-6、IL-8含量明显高于利巴韦林组,差异具有统计学意义(P<0.05)。结论:咳可灵可在一定程度上降低RVS感染小鼠肺泡灌洗液及肺组织中IL-6、IL-8水平,推测咳可灵合剂可作为预防及治疗RSV感染的潜在药物。

Objective:To explore the effect of kekeling mixture on the levels of interleukin-6(IL-6)and interleukin-8(IL-8)in mice infected with respiratory syncytial virus(RSV),so as to provide theoretical basis for the treatment of RSA.Methods:Totally 60 healthy male SD mice were randomly divided into 6 groups healthy group,model group,low,medium,high dose group and ribavirin group with 10 mice in each group.The general conditions of each group were observed and recorded after the establishment of RSV.Western blot(WB)and enzyme linked immunosorbent assay(ELISA)were used to detect the level of IL-6 and IL-8 in lung lavage fluid and lung tissue.Results:The RSV model was established successfully in the normal group because of the RSV infection.The alveolar epithelial cells in the normal group were relatively intact,the bronchi and pulmonary septum were intact,the alveolar epithelial cells in the RSV infected group were damaged,and the blood vessels were swollen and congested.The IL-6 and IL-8 water in the alveolar lavage fluid and lung tissue in the RSV infected group were higher than those in the normal group on average The difference was statistically significant(P<0.05);compared with the model group,the level of IL-6 and IL-8 in BALF and lung tissue decreased significantly after treatment(P<0.05),and the content of IL-6 and IL-8 in BALF and lung tissue in each group was significantly higher than that in ribavirin group(P<0.05).Conclusion:Kekelingcan reduce the level of IL-6 and IL-8 in BALF and lung tissue of RVs infected mice to a certain extent.It is speculated that kekeling mixture can be used as a potential drug to prevent and treat RSV infection.