摘要
The efficacy of intraperitoneal GY Y4137 therapy and intratesticular GY Y4137 therapy in an experimental rat model was investigated.Four groups were set up as the sham-operation group,torsion/detorsion(T/D)group,T/D plus intraperitoneal GY Y4137(G-IP)group,and T/D plus intratesticular GY Y4137(G-IT)group.In order to establish a testicular T/D model,the left testis was opcrated and the rotation reached 720°clockwise which lasted 1 h before reperfiusion.The G-IP group accepted 100 umol/kg of GY Y4137 intraperitoneally 30 min after testicular rotation,while the G-IT group was treated with the same dose by intratesticular injection.Six h after detorsion,the testis was collcted and subsequently asssed.The T/D group showed signifcant changes in histology and an enhancement in the level of oxidative stress and apoptosis compared to the sham-operation group.The expression of Caspase-3 and Bax turmed out to be strengthened by T/D and relatively decreased with GY Y4137 treatment in both the G-IP and G-IT groups.Moreover,the Bcl-2 expression was inhibited in the T/D group,and promoted by GYY4137 in the G-IP and G-IT groups.GYY4137,moderating these observed changes,displayed a more protective cffect with G-IT therapy than G-IP therapy.This study indicated that the efficacy of intratesticular therapy with GYY4137 is better than that of intraperitoneal therapy,which may provide a more valuable approach for testicular torsion therapy.
