补骨脂水提取物对去卵巢骨质疏松大鼠肾脏组织中TGF-β1/Smad4信号通路转导的影响

The Effects of Water Extract from Psoralea Corylifolia Linn on the TGFβ1/Smad4 Signaling Pathway in Ovariectomized Rats

目的:探讨补骨脂水提取物(PCE)对绝经后骨质疏松症的作用及分子机制。方法:采用双侧卵巢切除法建立绝经后骨质疏松(OVX)大鼠模型,评估OVX大鼠的体质量变化,采用双能X线吸收测量仪测量全身骨密度值,采用ELISA法检测血清碱性磷酸酶(ALP)和酸性磷酸酶(ACP)及雌激素E2分泌水平,采用苏木精-伊红(HE)染色检测股骨病理变化,采用RT-PCR和Western Blot法检测肾脏TGF-β1/Smad4信号分子mRNA和蛋白的表达水平。结果:补骨脂水提取物对绝经后骨质疏松症具有潜在的保护作用,能够显著减小术后大鼠体质量的增加(P>0.05),且增加OVX大鼠的全身骨密度值(P<0.05);同时其呈剂量依赖性下调ALP和ACP水平及上调E2水平(P<0.05),显著减小骨髓腔面积(P<0.05);除此之外,其呈剂量依赖性下调OVX大鼠肾脏组织中TGF-β1及Smad4的mRNA和蛋白表达水平(P<0.05)。结论:补骨脂水提取物对绝经后骨质疏松症具有潜在的保护作用,且与TGF-β1/Smad4信号通路介导骨代谢紊乱有关。

Objective:To investigate the effect of water extract from psoralea corylifolia linn(PCE) on postmenopausal osteoporosis and related molecular mechanisms.Methods:Postmenopausal osteoporosis(OVX) rat model was established by bilateral ovariectomy.The bone mineral density was measured by dual-energy X-ray absorptiometry.ELISA method was used to detect the secretion levels of ALP,ACP and E2 in rat serum.HE staining was used to detect the pathological changes of rat femur.RT-PCR and Western Blot were used to detect TGF-β1/Smad4 pathway-related molecules at mRNA and protein expression levels in rat kidney tissue.Results:PCE had a potential protective effect on postmenopausal osteoporosis,and it could significantly reduce the weight gain of postoperative rats and increased the whole body bone density value of OVX rats(P<0.05)).At the same time,it dose-dependently reduced the levels of ALP and ACP and up-regulated the level of E2(P<0.05),as well as significantly reducing the area of the bone marrow cavity(P<0.05).In addition,it dose-dependently down-regulated the kidney of OVX rats mRNA and protein expression levels of TGF-β1 and Smad4 in tissues(P<0.05).Conclusion:PCE relieves osteoporosis by inhibiting bone metabolism disorders mediated TGF-β1/Smad4 signaling pathway,and has potential effects in treating postmenopausal osteoporosis.