A supramolecular co-delivery strategy for combined breast cancer treatment and metastasis prevention

We herein propose a co-delivery approach where small interference RNA(siRNA)and anticancer chemotherapeutic drug are simultaneously loaded into a single delivery carrier for the combined treatment of breast cancer and metastasis prevention.The co-delivery vector is composed of chondroitin sulfate(CS)-coatedβ-cyclodextrin-polyethylenemine polymer,which is capable of loading paclitaxel(PTX)and siRNA simultaneously to form therapeutic nanocomplexes.The nanocomplex,termed as CPPTX-siCD146-CS,is demonstrated to have strong active targeting ability towards CD44-overexpresing breast cancer cells.Moreover,the co-delivery of PTX and siRNA not only effectively inhibits cancer cells proliferation and induces apoptosis,but also well prevents metastasis.Importantly,CP-PTX-siCD146-CS nanocomplexes exhibit stronger cytotoxic effects and anti-metastatic effects on MBA-MD-231 breast cancer cells,in comparison with PTX or siCD146 mono-treatment.The current study defines a potential therapeutic strategy for the combined breast cancer treatment and metastasis prevention from a codelivery perspective.