脱细胞生物敷料对烫伤创面愈合的影响

Effect of Acellular Dermis Matrix on Pigs Burning Wound Healing

目的研究脱细胞生物敷料对小型猪深Ⅱ度烫伤创面愈合的影响。方法巴马实验用小型猪24头,雌雄不限,体质量(15±3)kg通过铜锭制备大小为4 cm×6 cm的深Ⅱ度烫伤模型,随机分为两组,试验组使用脱细胞生物敷料,空白对照组使用脱脂棉纱布。术后1、2、3、4周统计创面愈合率和愈合时间,并进行临床观察、组织病理学观察及创面羟脯氨酸含量检测,分析创面愈合情况。结果试验过程中动物全部存活,且无创面感染情况发生,试验组术后创面干燥,2周结痂,3周脱痂并长出被毛;空白对照组术后1周创面表面局部有渗出,其余部位干燥,2周结痂,4周脱痂并长出被毛。创面愈合时间试验组为(20.83±0.75)d,空白对照组为(27.33±0.82)d。2周和3周时两组创面愈合率具有显著性差异。试验组术后3周皮肤各层结构清晰,烫伤面基本愈合,4周时皮肤各层结构完整;空白对照组皮肤各层结构和附件的形成与试验组相比皆滞后6 d左右。术后1周、2周和3周两组创面炎性细胞浸润情况和羟脯氨酸含量具有显著性差异。结论脱细胞生物敷料能够有效促进烫伤创面愈合,可缩短治疗周期。

Objective To study the effect of acellular dermis matrix on pigs with deep partial thickness burn.Method Twenty-four male and female miniature pigs,weighing(15±3)kg,were included.They were used to prepare the deep partial thickness burn model with the size of 4 cm×6 cm by copper ingots,then randomly divided them into two groups:experimental group using acellular dermis matrix and blank control group using absorbent cotton gauze.Detected the rate of wound healing,healing time,clinical and histopathological observation,hydroxyproline contents of wound in postoperative 1,2,3 and 4 weeks.Result During the experiment,all the animals survived,and no wound infection occurred.The wound surface of the experimental group was dried after operation,2 weeks incrustation,3 weeks decrustation and hair growth.In the blank control group,local exudation occurred on the wound surface at 1 weeks after operation,and the rest of the wound was dry,incrustation at 2 weeks,decrustation and covered with hair at 4 weeks.The time of wound healing was(20.83±0.75)d in the experimental group and(27.33±0.82)d in the blank control group.There was significant difference in wound healing rate between the two groups at 2 weeks and 3 weeks.The skin of the experimental group had clear structure at 3 weeks after surgery and the burn wound was basically healed,and after 4 weeks basic skin structure was intact.The formation of basic skin structure and appendages in the blank control group lagged by 6 days compared with the experimental group.There were significant differences in inflammatory cell infiltration and hydroxyproline contents of wound between the two groups at 1 weeks,2 weeks and 3 weeks.Conclusion Acellular dermis matrix could effectively promote the healing of burn wound,and shorten the treatment cycle.