荧光素类镁离子荧光增强型分子探针的制备及光谱性能研究

Preparation and spectral properties of fluoresce in magnesium ion fluorescent enhanced molecular probe

以荧光素与色酮-3-甲醛为原料合成了一种荧光增强型Mg^2+分子探针。采用核磁和质谱等对探针结构进行了表征。通过荧光光谱分析了探针的光谱性能。结果表明,探针对Mg^2+具有高选择性,可以特异性识别Mg^2+,而不受其他金属离子的影响。Mg^2+浓度在0. 6~24μmol/L范围内,与体系荧光强度呈现良好的线性关系,检出限为0. 435μmol/L。探针识别Mg^2+过程具有良好的可逆性。探针与Mg^2+以1:1比例进行配位的。探针对Mg^2+的识别机理是由于Mg^2+参与了与N,O原子的配位,使得分子内螺环发生了由闭环到开环的变化造成的。该探针在金属离子定量与分子检测等领域具有潜在的应用价值。

A fluorescent enhanced Mg^2+molecular probe was synthesized from fluorescein and chromone-3-carboxaldehyde.The probe structure was characterized by nuclear magnetic resonance and mass spectrometry.The spectroscopic properties of the probe were analyzed by fluorescence spectra.The results showed that the probe had high selectivity to Mg^2+,and can identify Mg^2+specifically without being affected by other metal ions.When Mg^2+concentration was in the range of 0.6-24μmol/L,it had a good linear relationship with fluorescence intensity of the system,and the detection limit was 0.435μmol/L.The process of Mg^2+identification by probe has good reversibility.The probe coordinates with Mg^2+in 1:1 ratio.The recognition mechanism of Mg^2+by probe is the coordination of Mg^2+with N and O atoms,resulting in the change ofmolecular internal spiral ring from closed-loop to open-loop.The probe has potential application in metal ionquantification and molecular detection.