罗氏蝴蝶兰的无菌播种与快速繁殖

Aseptic seeding and rapid propagation of Phalaenopsis lobbii

罗氏蝴蝶兰(Phalaenopsis lobbii)是珍稀濒危植物,被列为国家极小种群野生植物。组培快繁技术是保护植物的有效方法。本文以授粉后发育成熟的罗氏蝴蝶兰种子为材料进行无菌播种研究,探究罗氏蝴蝶兰快繁体系的最适条件。实验结果表明:罗氏蝴蝶兰种子萌发不需要黑暗处理,最适合种子萌发的培养基为1/4MS+0.1%活性炭+70 g·L^-1香蕉汁,种子萌发率为77.20%;低浓度6-BA和较高浓度NAA对丛生芽诱导有利,最适诱导培养基为1/3MS+0.5 mg·L^-1 6-BA+2.0 mg·L^-1 NAA+0.1%活性炭+70 g·L^-1香蕉汁;适合生根壮苗的培养基为1/3MS+70 g·L^-1香蕉汁或1/3MS+70 g·L^-1马铃薯汁。通过无菌播种研究,成功获得了一批罗氏蝴蝶兰幼苗。

Phalaenopsis lobbii is a rare and endangered plant, which is included in the wild plant species with extremely small populations in China. Tissue culture and rapid propagation is an effective method to protect the plant. In this study, we used the mature seeds of P. lobbii after pollination as materials to explore the optimal condition of the aseptic seeding. The results showed that the seed germination didn’t need dark treatment. The most suitable medium for the seed germination was 1/4 MS + 0.1% activated carbon + 70 g·L^-1 banana juice, and the seed germination rate was 77.20%. Low concentration of 6-BA and high concentration of NAA were beneficial to the induction of multiple shoot, and the optimal medium was 1/3 MS + 0.5 mg·L^-1 6-BA + 2.0 mg·L^-1 NAA + 0.1% activated carbon + 70 g·L^-1 banana juice. Added juices of potato or banana in the medium was helpful to the plant growth, and the suitable medium was 1/3 MS + 70 g·L^-1 potato juice or 1/3 MS + 70 g·L^-1 banana juice. Through the aseptic seeding research, we successfully obtained a batch of tissue culture seedlings of P. lobbii.