2011-2017年大连市输入性卵形疟原虫感染病例的实验室诊断

Laboratory diagnosis on cases of imported Plasmodium ovale infection in Dalian from 2011 to 2017

目的分析大连市2011-2017年7例输入性卵形疟原虫感染病例的病原、抗原及核酸检测结果,以提高卵形疟原虫诊断的准确率和效率。方法收集7例疑似疟疾患者的血样,对血样进行血涂片镜检、快速疟疾诊断试纸条检测(RDT)及荧光定量PCR检测。结果血涂片镜检结果显示7例患者均感染卵形疟原虫。RDT检测结果显示2例阴性,另外5例为泛疟原虫抗原阳性。荧光定量PCR结果显示7例患者均检出疟原虫属和卵形疟原虫核酸。结论综合镜检、RDT及荧光定量PCR检测结果确定该7例患者均为卵形疟原虫感染。

Objective To analyze the laboratory results in detecting the parasite morphology,antigen and DNA in seven imported cases of Plasmodium ovale infection in order to improve the precision and efficiency of diagnosis for the disease.Methods Seven blood samples were collected and detected by using microscopy,Rapid Diagnostic Test(RDT)and Real-time fluorescent quantitative PCR.Results Microscopic examination of blood smear showed that all 7 patients were infected with Plasmodium ovale.RDT eliminated 5 cases of Plasmodium spp infection from the 7 patients.The other 2 patients were negative.The DNA of Plasmodium spp and Plasmodium ovale were detected from all the seven blood samples with Real-time fluorescent quantitative PCR.Conclusion All the seven cases of Plasmodium ovale infection were identified by combined confirmation with microscopy,RDT and Real-time fluorescent quantitative PCR.