磷酸化蛋白激酶B和造血祖细胞激酶1蛋白表达与乳腺癌患者预后的关系研究

Study on the relationship between the expression of phosphorylated protein kinase B and hematopoietic progenitor cell kinase 1 protein and the prognosis of breast cancer patients

目的探讨磷酸化蛋白激酶B(p-Akt)和造血祖细胞激酶1(HPK1)表达与乳腺癌患者预后的关系。方法选取102例乳腺癌组织标本和对应的癌旁组织标本进行实验。用试剂盒检测p-Akt和HPK1蛋白的表达情况,并分析其与患者生存情况的关系。结果乳腺癌组织和癌旁组织的p-Akt阳性表达率分别为54.90%(56例/102例)和16.67%(17例/102例),HPK1阳性表达率分别为40.20%(41例/102例)和82.35%(84例/102例),差异均有统计学意义(均P<0.05)。乳腺癌组织的p-Akt与HPK1表达水平呈负相关(rs=-0.463,P<0.05)。p-Akt阳性组和p-Akt阴性组的平均总生存时间分别为69.40和84.54个月,HPK1阳性组和HPK1阴性组的平均总生存时间分别为84.24和68.88个月,差异均有统计学意义(均P<0.05)。结论乳腺癌组织的p-Akt表达显著上调,而HPK1表达显著下调,且其可能与患者预后有关。

Objective To investigate the relationship between the expressions of phosphorylated protein kinase B(p-Akt)and hematopoietic progenitor cell kinase 1(HPK1)and the prognosis of breast cancer patients.Methods A total of 102 breast cancer tissue samples and corresponding adjacent tissue samples were selected for the experiment.The expressions of p-Akt and HPK1 protein were detected by kit,and the relationship between the protein expression and the survival condition of the patients were analyzed.Results The positive rates of p-Akt in breast cancer tissues and paracancerous tissues were 54.90%(56 cases/102 cases)and 16.67%(17 cases/102 cases),the positive rates of HPK1 were 40.20%(41 cases/102 cases)and 82.35%(84 cases/102 cases).The differences were statistically significant(all P<0.05).There was a negative correlation between the expression of p-Akt and HPK1 in breast cancer(rs=-0.463,P<0.05).The mean total survival time of p-Akt positive group and p-Akt negative group was 69.40 and 84.54 months,the mean total survival time of HPK1 positive group and HPK1 negative group was 84.24 and 68.88 months.The differences were statistically significant(all P<0.05).Conclusion The expression of p-Akt in breast cancer is significantly up-regulated,while the expression of HPK1 is significantly down-regulated,which may be related to the prognosis of patients.