桔梗皂苷D对子宫内膜癌细胞凋亡和侵袭的影响

Effect of platycodin D on apoptosis and invasion of endometrial cancer cells

目的研究桔梗皂苷D(PD)在子宫内膜癌细胞增殖,凋亡,迁移和侵袭中的作用和分子机制。方法转染前,将子宫内膜癌细胞分为4组,空白组和低,中,高剂量PD组。转染时,将细胞分为空白组,中剂量PD组,中剂量PD+si-con组,中剂量PD+si-KLF4组。空白组仅予以常规培养,中剂量PD组予以12μmol·L^-1 PD,中剂量PD+si-con组予以12μmol·L^-1 PD+si-con,中剂量PD+si-KLF4组予以12μmol·L^-1 PD+si-KLF4,各组均处理48 h。用Transwell法检测细胞迁移和侵袭,流式细胞术检测细胞凋亡。结果转染前,空白组,低,中,高剂量PD组细胞迁移数分别为(187.28±12.77),(142.18±8.63),(89.32±5.21),(52.64±4.40)个,细胞侵袭数分别为(85.61±8.78),(67.83±6.55),(45.89±3.50),(29.37±3.39)个,细胞凋亡率分别为(6.27±0.35)%,(12.31±0.68)%,(16.81±1.24)%,(23.69±1.52)%,中、高剂量PD组的上述指标与空白组比较,差异均有统计学意义(均P<0.05)。转染后,空白组,中剂量PD组,中剂量PD+si-con组,中剂量PD+si-KLF4组的细胞迁移数分别为(194.56±14.32),(92.83±8.47),(85.88±7.49),(139.84±9.76)个,细胞侵袭数分别为(83.79±9.75),(41.95±7.43),(39.81±6.85),(95.32±8.78)个,细胞凋亡率分别为(6.27±0.35)%,(18.48±0.94)%,(19.81±1.04)%,(10.67±1.39)%,中剂量PD组的上述指标与空白组比较,中剂量PD+si-KLF4组的上述指标与中剂量PD+si-con组比较,差异均有统计学意义(均P<0.05)。结论 PD通过调控KLF4表达抑制子宫内膜癌细胞迁移和侵袭,并诱导其凋亡。

Objective To study the role and molecular mechanism of platycodin D(PD)in the proliferation,apoptosis,migration and invasion of endometrial cancer cells.Methods Endometrial cancer cells were divided into four groups,blank group,low-,medium-and high-dose PD groups before transfection.KLF4 negative control plasmid and si-KLF4 were transfected into PD-treated endometrial cancer cells,respectively,named blank group,low-,medium-,high-dose PD groups,medium-dose PD+si-con group,medium-dose PD+si-KLF4 group.Untreated cells were named blank group,the medium-dose PD group was treated with 12μmol·L^-1 PD,the medium-dose PD+si-con group was treated with 12μmol·L^-1 PD+si-con,and the medium-dose PD+si-KLF4 group was treated with 12μmol·L^-1 PD+si-KLF4,each group was treated for 48 h.Cell migration and invasion were detected by Transwell method,and apoptosis was detected by flow cytometry.Results Before transfection,the number of cell migration in the blank group,low-,medium-and high-dose PD groups were 187.28±12.77,142.18±8.63,89.32±5.21 and 52.64±4.40,respectively;the number of cell invasion were 85.61±8.78,67.83±6.55,45.89±3.50,29.37±3.39,respectively;the cell apoptotic rate were(6.27±0.35)%,(12.31±0.68)%,(16.81±1.24)%,(23.69±1.52)%,respectively;the differences of the three indices between the medium-dose PD group,the high-dose PD group and the blank group were statistically significant(P<0.05).After transfection,the number of cell migration in blank,medium-dose PD,medium-dose PD+si-con,and medium-dose PD+si-KLF4 were 194.56±14.32,92.83±8.47,85.88±7.49,and 139.84±9.76,respectively;the number of cell invasion were 83.79±9.75,41.95±7.43,39.81±6.85,95.32±8.78,respectively;the apoptosis rate were(6.27±0.35)%,(18.48±0.94)%,(19.81±1.04)%,(10.67±1.39)%,respectively;the differences of the three indices between medium-dose PD group with blank group,and medium-dose PD+si-KLF4 group with medium-dose PD+si-con group were statistically significant(all P<0.05).Conclusion PD inhibits the migration and invasion,and induces apoptosis of endometrial cancer cells by regulating KLF4 expression.