玉女煎含药血清对胰岛β细胞株INS-1增殖和凋亡的影响研究

The Effect of Drug-containing Serum of Yunvjian on Proliferation and Apoptosis of Islet β Cell Line INS-1

目的:探讨玉女煎含药血清对胰岛β细胞株INS-1增殖和凋亡的影响。方法:用SPF级Wistar大鼠制备含药血清;MTT法测INS-1的生长曲线,选取浓度分别为5%、10%、15%和20%的玉女煎含药血清作用于对数生长期的INS-1细胞,分别作用24h、48h和72h,采用MTT法检测细胞增殖;选取5%、10%、20%的玉女煎含药血清作用于对数生长期的INS-1细胞,分别作用24h和48h,采用流式细胞术检测细胞的凋亡。结果:从生长曲线可知,INS-1的对数生长期在第3~6天;与对照组比较,10%玉女煎含药血清在所测时间段均能促进细胞增殖(P<0.05),5%和15%含药血清作用24h促进细胞增殖(P<0.05)。与对照组比较,玉女煎各组作用24h后均显著抑制细胞凋亡(P<0.01或P<0.05),作用48h后,10%组继续显著抑制细胞凋亡(P<0.05)。结论:玉女煎含药血清能促进INS-1细胞的增殖并减少INS-1细胞的凋亡。

Objective:To investigate the effect of drug-containing serum of Yunvjian on proliferation and apoptosis of isletβcell line INS-1.Methods:The growth curve of INS-1 was measured by MTT method;the concentration of 5%,10%,15%and 20%of drug-containing serum of Yunvjian was selected to act on INS-1 cells in the logarithmic growth phase,respectively for 24 h,48 hand 72 h,MTT assay was used to detect the proliferation of INS-1 cells;the concentration of 5%,10%and 20%of drug-containing serum of Yunvjian was selected to act on INS-1 cells in the logarithmic growth phase,respectively for 24 hand 48 h,flow cytometry was used to detect the apoptosis of INS-1 cell.Results:Apparent from growth curves,the logarithmic period of INS-1 was on the 3rd to 6th day;compared with the control group,10%drug-containing serum of Yunvjian can promote cell proliferation in the measured time period(P<0.05),5%and 15%drug-containing serum group can promote cell proliferation at 24 h(P<0.05);each group of drug-containing serum of Yunvjian significantly inhibited apoptosis after 24 h(P<0.01 or P<0.05).After 48 h,10%group continued to significantly inhibit apoptosis(P<0.05).Conclusion:The drug-containing serum of Yunvjian can promote the proliferation of INS-1 cells and decrease the apoptosis of INS-1 cells.