HPLC法快速测定五种含厚朴制剂中和厚朴酚与厚朴酚的含量

Determination of Honokiol and Magnolol in Magnolia Officinalis Preparations by HPLC

目的:建立一个同时快速测定五种含厚朴制剂(木香顺气丸、清肝利胆胶囊、香连化滞丸、泻痢消丸和泻痢消片)中和厚朴酚与厚朴酚含量的HPLC方法。方法:色谱柱:Welch Ultimate LP-C18(250mm×4.6mm,5μm),流动相:甲醇-0.05%磷酸溶液(75∶25),柱温:30℃,流速:1.0mL·min-1,检测波长:290nm。结果:和厚朴酚、厚朴酚与相邻峰的分离度符合要求,方法专属性好,质量浓度范围分别为1.32~131.67μg·mL-1(R2=1.000 0)和2.93~293.24μg·mL-1(R2=0.999 8);平均加样回收率(n=6)分别为98.6%~99.6%,RSD 0.8%~1.7%;98.7%~100.1%,RSD 0.5%~1.6%,结果较好。仪器进样精密度、方法重复性、溶液稳定性均符合要求。结论:本法简单、快速、准确,适用于含厚朴制剂中和厚朴酚与厚朴酚的含量测定,可推广应用于含厚朴制剂快检工作。

Objective:To establish a HPLC method for the determination of honokiol and magnolol in magnolia officinalis preparations.Methods:The analysis was performed on a Welch Ultimate LP-C18(250 mm×4.6 mm,5μm)with the mobile phase consisting of methanol-0.05%phosphoric acid(75∶25)at the flow rate of 1.0 mL·min-1,the column temperature was 30℃,and the detection wavelength was 290 nm.Results:Honokiol and magnolol peak was successfully separated from each other,with good specificity.The linear ranges of calibration curves,respectively,were 1.32~131.67μg·mL-1(r=1.0000)and 2.93~293.24μg·mL-1(r=0.9999).The average recoveries(n=6)were 98.6%~99.6%(RSD=0.8%~1.7%),98.7%~100.1%(RSD=0.5%~1.6%).The precision,repeatability and stability were compliance with requirements.Conclusion:The established method is simple,rapid,accurate,and suitable for the determination of honokiol and magnolol in magnolia officinalis preparations,and should to be promoted.