细胞毒性T细胞、巨噬细胞和调节性T细胞在复发性宫颈癌患者中的免疫特征分析

Immunological characteristics of cytotoxic T cells, macrophages and regulatory T cells in patients with recurrent cervical cancer

目的探讨毒性T细胞(CTL)、巨噬细胞(TAMs)和调节性T细胞(Tregs)在复发性宫颈癌患者中的免疫特征。方法收集复发性宫颈癌细胞及癌旁细胞,利用白细胞分化抗原8(CD8)、簇分化抗原68(CD68)、叉头翼状螺旋转录因子(FoxP3)分别表示CTL、TAMs和Tregs在复发性宫颈癌中的免疫特性,程序性细胞死亡蛋白1(PD-1)和程序性死亡配体1(PD-L1)表示癌细胞自身免疫,通过多重免疫荧光板检测细胞中CD8^+、CD68^+、FoxP3^+表达量,RNA免疫共沉淀法分析其在复发性宫颈癌细胞中的免疫特性。结果在肿瘤及肿周组织中CD8^+、CD68^+、FoxP3^+表达均较高,但肿周组织中FoxP3^+更高;高表达的FoxP3^+与CD8^+、CD68^+有显著相关性,可促进CD8^+、CD68^+凋亡而上调PD-L1、PD1表达。结论Tregs可对肿瘤组织中的CTL和TAM进行调节,可提高宫颈癌细胞的免疫应答。

This study was preformed to investigate the immunological characteristics of cytotoxic T cells(CTLs),macrophages(TAMs)and regulatory T cells(Tregs)in patients with recurrent cervical cancer.The cancer cells and adjacent normal cells were collected for detecting the cluster of differentiation 8(CD8^+),cluster of differentiation 68(CD68^+)and forkhead/winged helix transcription factor 3(FoxP3^+),as reflections of the characteristics of CTLs.TAMs and Treg.Meanwhile,the programmed cell death protein 1(PD-1)and programmed death ligand 1(PD-L1)were used to indicate cancer cell autoimmunity.Data showed that CD8^+,CD68^+,and FoxP3^+were highly expressed in the cancer tissues and adjacent tissues.Moreover,the elevated expression of FoxP3^+could promote the apoptosis of CD8^+and CD68^+,thus up-regulating the expression of PD-L1 and PD1,indicating that Tregs can regulate CTL and TAM in tumor tissues and improve the immune response of cervical cancer cells.