瘦素受体Q223R基因多态性与系统性红斑狼疮发病关联的Meta分析

Meta-analysis on the association of leptin receptor Q223R polymorphism with the susceptibility to systemic lupus erythematosus

目的瘦素受体(leptin receptor,LEPR)Q223R(Gln>Arg)基因多态性与系统性红斑狼疮(systemic lupus erythematosus,SLE)发病关联尚存在较大争议。该研究采用Meta分析方法,综合评价LEPR Q223R基因多态性与SLE易感性关联。方法使用Medline(PubMed)、Web of Science、中国知网(CNKI)、万方数字化期刊全文数据库等数据库,全面检索有关LEPR Q223R基因多态性与SLE发病易感性相关的病例对照研究,检索时间至2020年4月。提取SLE与健康对照A/G等位基因频率、AA/AG/GG基因型数据,以比值比(odds ratio,OR)及95%可信区间(confidence interval,CI)作为合并效应量指标,分别分析等位基因、基因型与SLE发病关联。定量分析各研究间的异质性,采用Begg和Egger检验评估发表偏倚。结果共检索4篇研究的7项病例对照研究,Meta分析共纳入SLE患者9052例,健康对照8146例。结果显示,LEPR Q223R A/G基因多态性与SLE易感性关联无统计学意义(P>0.05),A等位基因与SLE发病关联的合并OR值为1.03(95%CI:0.92~1.14);基因型显性(AA+AG比GG)、隐性模型(AA比AG+GG)均提示LEPR Q223R A/G基因多态性与SLE发病无关联,合并OR(95%CI)分别为0.88(0.15~5.37)、1.13(0.37~3.49);结果还显示,不同人群LEPR Q223R基因型分布存在差异,研究间异质性较大。结论现有证据尚不足以表明,LEPR Q223R A/G基因多态性与SLE发病易感性存在关联。

Objective The association between leptin receptor(LEPR)Q223R(Gln>Arg)gene polymorphism and systemic lupus erythematosus(SLE)remains controversial.In this study,a meta-analysis was used to comprehensively evaluate the association between LEPR Q223R gene polymorphism and SLE susceptibility.Methods Case control studies on the relationship between LEPR Q223R gene polymorphism and SLE susceptibility were comprehensively searched by Medline(PubMed),Web of Science,CNKI,Wanfang digital journal full-text database,etc.,and the search time was up to April 2020.The data of A/G allele frequency and AA/AG/GG genotype in SLE patients and healthy controls were extracted,the odds ratio(OR)value and 95%confidence interval(CI)were used as the combined effect-size indicators to analyze the correlation between allele,genotype and SLE risk.The heterogeneity among studies was analyzed quantitatively,and the publication bias was evaluated by Begg and Egger’s test.Results A total of 7 case-control studies from 4 studies were retrieved.A total of 9052 patients with SLE and 8146 healthy controls were included in the meta-analysis.The results showed that there was no significant association between LEPR Q223R A/G gene polymorphism and SLE susceptibility,and the OR of A allele in LEPR Q223R gene locus associated with SLE risk was 1.03(95%CI:0.92-1.14).The dominant(AA+AG vs GG)and recessive(AA vs AG+GG)models both suggested that LEPR Q223R A/G gene polymorphism was not associated with SLE,and the combined OR(95%CI)was 0.88(0.15-5.37)and 1.13(0.37-3.49),respectively.The results also showed that the distribution of LEPR Q223R genotype was different among different populations,and the inter-study heterogeneity was large.Conclusion The existing evidence is insufficient to indicate that there is an association between LEPR Q223R A/G gene polymorphism and SLE susceptibility,which needs to be confirmed by further studies.