血清miRNA表达谱在肝细胞癌中的差异性研究

The differential research of serum miRNA expression spectrum in hepatocellular carcinoma

[目的]探讨肝细胞癌、肝硬化患者与正常人血清miRNA表达水平的差异性;筛选出肝癌中差异较明显的血清miRNA谱。[方法]分别收集4例肝癌患者、3例肝硬化患者和3例同期正常体检人员的血清,采用Qiagen miRNA PCR Array芯片法筛查肝癌、肝硬化患者和正常人差异表达的血清miRNA。[结果]初步筛查出了差异表达的血清miRNA,与正常人比较,以相差2倍为分界,肝癌血清miRNA共128个下调,98个上调;肝硬化血清miRNA共131个下调,74个上调;其中,肝癌和肝硬化患者血清miRNA同时下调的有76个,同时上调的有53个。与肝硬化比较,以相差2倍为分界,肝癌血清miRNA共108个下调,103个上调;结合文献、P值及CT值进一步筛选出差异最有意义的7个与肝癌相关的血清miRNA(miR-373-5p,miR-1290,miR-4516,miR-134-5p,miR-144-5p,miR-125a-5p,miR-126-3p)。[结论]差异表达的miRNA谱与肝癌的发生、发展有一定的相关性。

[Objective]To investigate the difference of serum miRNA expression between patients with hepatocellular carcinoma、cirrhosis and normal subjects.[Methods]We collected serum samples from 4 patients with hepatocellular carcinoma,3 patients with cirrhosis and 3 cases with normal physical examination during the same period.Serum miRNA expression were screened by Qiagen miRNA PCR Array microarray.[Results] The differentially expressed of serum miRNA were initially screened.Compared with normal(2-fold difference as the dividing line),changes of serum miRNA were detected in hepatocellular carcinoma(a total of 128 down-regulated and 98 up-regulated)and in cirrhosis(a total of 131 down-regulated and 98 up-regulated).Compared with cirrhosis(2-fold difference as the dividing line),changes of serum miRNA were detected in hepatocellular carcinoma(a total of 108 down-regulated and 103 up-regulated).Combined with literature,p value and CT value,7 serum miRNA with the most significant differences related to hepatocellular carcinoma were further screened(miR-373-5 p,miR-1290,miR-4516,miR-134-5 p,miR-144-5 p,miR-125 a-5 p,miR-126-3 p).[Conclusion] There are correlation between the miRNA expression spectrum with the occurrence and development of hepatocellular carcinoma.