摘要
以樟叶越桔组培苗为试验材料,研究不同植物生长调节剂对组培苗长势及其主要次生代谢产物6′-O-咖啡酰熊果苷生产的影响。结果表明:CK的WPM培养基组培苗叶片和茎段中的6′-O-咖啡酰熊果苷含量分别为(1.78±0.14)%和(0.75±0.09)%;在WPM+0.2 mg/L NAA培养基中樟叶越桔组培苗长势最好,但叶片和茎段中的6′-O-咖啡酰熊果苷含量分别仅为(1.59±0.01)%和(0.83±0.005)%;在WPM+2.0 mg/L ZT+0.05 mg/L NAA培养基中叶片和茎段中6′-O-咖啡酰熊果苷含量达到最高,分别为(10.77±0.31)%和(3.67±0.09)%,但组培苗的生长受到抑制,长势较弱且叶片枯黄;在WPM+0.5 mg/L ZT+0.2 mg/L NAA培养基中组培苗长势良好且6′-O-咖啡酰熊果苷含量相对较高,在叶片和茎段中分别为(7.33±0.18)%和(2.00±0.05)%,适宜于樟叶越桔组培苗的生长和6′-O-咖啡酰熊果苷的生产。
The effects of different plant growth regulators on the seedlings growth and production of the main secondary metabolite 6'-O-Caffeoylarbutin(CA)were investigated by using the tissue culture seedlings of Vaccinium dunalianum.As the results,the CA contents in the leaves and stem segments in WPM medium(CK)were(1.78±0.14)%and(0.75±0.09)%,respectively.In the medium of WPM+0.2 mg/L NAA,the seedlings grew best,however,their CA contents in the leaves and stem segments were only(1.59±0.01)%and(0.83±0.005)%,respectively.When the cultivation under a medium of WPM+2.0 mg/L ZT+0.05 mg/L NAA,the CA contents in the leaves and stem segments was the highest(10.77±0.31)%and(3.67±0.09)%,respectively,while the growth situation of the seedlings was weaker with yellow withered leaves.The cultivation condition with good growth situation of seedlings and higher CA contents(7.33±0.18)%and(2.00±0.05)%,respectively in the leaves and stem segments was the medium of WPM+0.5 mg/L ZT+0.2 mg/L NAA,which is more suitable for the seedlings growth and CA production.
作者
尚俊可
唐军荣
罗旭璐
付羚
刘云
赵平
Shang Junke;Tang Junrong;Luo Xulu;Fu Ling;Liu Yun;Zhao Ping(Key Laboratory of National Forestry and Grassland Administration for Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650233,China;Collaborative Innovation Center of Forest Resources Breeding and Utilization in Yunnan,Kunming Yunnan 650233,China;Yunnan Forestry Technological College,Kunming Yunnan 650224,China)
出处
《西南林业大学学报(自然科学)》
CAS
北大核心
2020年第4期157-162,共6页
Journal of Southwest Forestry University:Natural Sciences
基金
云南省农业基础研究联合专项重点项目(2017FG001(-016))资助
中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室开放基金项目(P2017-KF10)资助
国家自然科学基金项目(21462040)资助。