不同植物生长调节剂对樟叶越桔组培苗6′-O-咖啡酰熊果苷生产的影响

Effects of Different Plant Growth Regulators on the Production of 6'-O-Caffeoylarbutin in Tissue Culture Seedlings of Vaccinium dunalianum

以樟叶越桔组培苗为试验材料,研究不同植物生长调节剂对组培苗长势及其主要次生代谢产物6′-O-咖啡酰熊果苷生产的影响。结果表明:CK的WPM培养基组培苗叶片和茎段中的6′-O-咖啡酰熊果苷含量分别为(1.78±0.14)%和(0.75±0.09)%;在WPM+0.2 mg/L NAA培养基中樟叶越桔组培苗长势最好,但叶片和茎段中的6′-O-咖啡酰熊果苷含量分别仅为(1.59±0.01)%和(0.83±0.005)%;在WPM+2.0 mg/L ZT+0.05 mg/L NAA培养基中叶片和茎段中6′-O-咖啡酰熊果苷含量达到最高,分别为(10.77±0.31)%和(3.67±0.09)%,但组培苗的生长受到抑制,长势较弱且叶片枯黄;在WPM+0.5 mg/L ZT+0.2 mg/L NAA培养基中组培苗长势良好且6′-O-咖啡酰熊果苷含量相对较高,在叶片和茎段中分别为(7.33±0.18)%和(2.00±0.05)%,适宜于樟叶越桔组培苗的生长和6′-O-咖啡酰熊果苷的生产。

The effects of different plant growth regulators on the seedlings growth and production of the main secondary metabolite 6'-O-Caffeoylarbutin(CA)were investigated by using the tissue culture seedlings of Vaccinium dunalianum.As the results,the CA contents in the leaves and stem segments in WPM medium(CK)were(1.78±0.14)%and(0.75±0.09)%,respectively.In the medium of WPM+0.2 mg/L NAA,the seedlings grew best,however,their CA contents in the leaves and stem segments were only(1.59±0.01)%and(0.83±0.005)%,respectively.When the cultivation under a medium of WPM+2.0 mg/L ZT+0.05 mg/L NAA,the CA contents in the leaves and stem segments was the highest(10.77±0.31)%and(3.67±0.09)%,respectively,while the growth situation of the seedlings was weaker with yellow withered leaves.The cultivation condition with good growth situation of seedlings and higher CA contents(7.33±0.18)%and(2.00±0.05)%,respectively in the leaves and stem segments was the medium of WPM+0.5 mg/L ZT+0.2 mg/L NAA,which is more suitable for the seedlings growth and CA production.