中国南海海绵Ircinia sp.中化学成分的研究

Studies on chemical constituents of the marine sponge Ircinia sp.from the South China Sea

目的:对采自广西涠洲岛海域海绵Ircinia sp.进行化学研究以获得结构新颖的新化合物。方法:运用高速逆流色谱、凝胶渗透色谱、硅胶柱色谱和半制备型高效液相色谱等多种技术分离纯化,再通过氢谱、碳谱和质谱等谱学解析方法鉴定出单体成分的结构。结果:从海绵Ircinia sp.中分离鉴定出5个化合物,分别命名为cholest-7-en-6R-methoxy-3S,5R-diol(1),cholest-7-en-3S,5R,6R-triol(2),cholest-7,22-dien-6R-methoxy-3S,5R-diol(3),鲨肝醇(4)和邻苯二甲酸正丁酯(5)。结论:化合物1~3为首次从该属海绵中分离得到。

Objective:Chemical components of the marine sponge Ircinia sp.from the South China Sea were studied in order to obtain new compounds with novel structures.Methods:HSCCC,GPC,Silica gel column chromatography and semi-preparative HPLC were preformed to isolate and purify the dichloromethane extract of the marine sponge Ircinia sp.The structures of compounds were identified by spectroscopic methods such as 1H NMR,13C NMR,2D NMR and MS.Results:Five compounds were obtained and identified,including three steroids,namely cholest-7-en-6R-methoxy-3S,5R-diol(1),cholest-7-en-3S,5R,6R-triol(2)and cholest-7,22-dien-6R-methoxy-3S,5R-diol(3),one diol,namely batyl alcohol(4),and one lipid,namely dibutyl phthalate(5).Conclusion:Compounds 1-3 are obtained for the first time from the genus Ircinia.