基于Hi-C数据的染色体三维分析计算方法

Three-Dimensional Analysis and Calculation Methods of Chromosome Based on Hi-C Data

真核生物的染色质在细胞核中高度折叠且有序排列,这种三维结构对基因转录和DNA复制等细胞功能发挥至关重要。近年来Hi-C数据的涌现,使得研究染色质三维交互作用及其空间结构成为了可能。自2009年Hi-C被开发以来,已经出现了许多用于处理Hi-C数据的生物信息学工具,从原始序列比对到接触矩阵的可视化,均提供了系统的Hi-C数据处理过程或解决某一个特定问题的方法。本研究全面介绍了传统的Hi-C数据处理工作流程,以及近十年来新兴的Hi-C数据处理工具以及其未来发展方向。

The chromatin of eukaryotes is highly folded and ordered in the nucleus,and this three-dimensional structure plays an important role in cellular functions such as gene transcription and DNA replication.Recently,with the emergence of Hi-C data,it has become possible to study the three-dimensional interaction of chromatin and its spatial structure.Since the development of Hi-C in 2009,many bioinformatics tools for processing Hi-C data have emerged,from the original sequence alignment to the visualization of the contact matrix,providing systematic Hi-C data processing or a solution to a particular problem.This article provides a comprehensive introduction to the traditional Hi-C data processing flow,as well as the emerging Hi-C data processing tools in the past decade and their future direction.