武夷菌素对土壤微生物群落及抗生素抗性基因的影响

Impact of Wuyiencin on Microbial Community and Antibiotic Resistance Genes of Soil

抗生素抗性基因(antibiotic resistance genes,ARGs)作为一种新型环境污染物近年来受到广泛关注。目前关于抗生素的环境污染研究主要集中于医疗和养殖业,对植物保护领域的农用抗生素环境污染研究很少。武夷菌素是一种环保、高效、广谱的农用抗生素,在农业生产中得到了广泛应用,对农作物真菌性病害具有良好的防治效果。本研究分别选取了未使用武夷菌素和使用武夷菌素的蔬菜大棚中的土壤,通过高通量测序分析了土壤中微生物群落结构,发现两份土壤中主要的微生物群落种类没有发生明显改变,但是优势菌群的丰度有显著差异。通过荧光定量PCR技术,对18个典型的抗生素抗性基因进行了检测,发现aadA、aac(3)-Ⅱ、strA、strB、aacA4、tetX、sulI和intI18个基因在两份土壤中的绝对含量和丰度均有显著差异,表明武夷菌素对土壤中微生物的群落结构及抗生素抗性基因的绝对含量和丰度均会造成一定影响。本研究为评估武夷菌素的环境安全性及合理正确使用武夷菌素提供了理论依据,也为其它农用抗生素的相关研究提供了借鉴。

Antibiotic resistance genes(ARGs)were wildly concerned in recent years as a new type of environmental pollutant.At present,the research on environmental pollution of antibiotics mainly focuses on medical and aquaculture,and there are few studies on the environmental pollution of agricultural antibiotics in the field of plant protection.Wuyiencin is a kind of environmentally-friendly,high-efficiency,broad-spectrum agricultural antibiotic.It has been widely used in agricultural production and has good control effects on crop fungal diseases.In this research,the soils in vegetable greenhouses were selected as materials with and without spraying Wuyiencin,and the microbial community structure in the soil was analyzed by high-throughput sequencing.It was found that the main microbial community species in the two soils did not change significantly.However,the abundance of dominant microbial was significantly different.Eighteen typical antibiotic resistance genes were detected by real-time PCR,and it was found that the absolute content and abundance of the eight genes aadA,aac(3)-II,strA,strB,aacA4,tetX,sulI and intI1 were significantly different in the two soils,indicating that Wuyiencin could cause certain effects on microbial community structure and absolute content and abundance of antibiotic resistance genes in soil.This study provided a theoretical basis for the assessment of environmental safety of Wuyiencin and the rational and correct use of it,as well as a reference for other related research on agricultural antibiotics.