摘要
本研究探讨西红花苷提前干预用药对急性高海拔低氧条件下大鼠脑海马组织的影响。以西红花苷药效机制为研究内容,通过组织病理及透射电镜超微结构形态学观察,RT-PCR和Western blotting检测PGC-1α、TFAM、Bcl-2、Bax、Caspase-3在mRNA和蛋白水平表达,TUNEL凋亡检测海马神经元凋亡水平。研究发现,在低氧模型组海马组织在第1天、第3天、第5天、第7天细胞结构有不同程度紊乱,线粒体损伤,以第1天、第3天最为显著,而西红花苷组线粒体损伤明显改善;PGC-1α、TFAM、Bcl-2相对表达量比较发现西红花苷组在mRNA和蛋白水平表达在第1天、第3天和第5天明显高于低氧模型组(p<0.05),而第7天两组相对表达量差别无统计学意义(p>0.05);比较Bax、Caspase-3在第1天、第3天和第5天m RNA和蛋白水平相对表达量发现西红花组相明显低于低氧模型组(p<0.05),而第7天两组相对表达量差别无统计学意义(p>0.05);TUNEL检测发现脑海马CA1区神经元凋亡率与低氧模型比较西红花苷组在第1天、第3天、第5天比较均较低(p<0.05),表明西红花苷通过抗线粒体损伤及凋亡具有保护急性高海拔低氧大鼠脑海马的药理作用,为研究高原病的防治提供理论依据。
To observe the effect of crocin in advance of the medication on the hippocampus under hypoxia at high altitude in rats.The morphological changes of hippocampal neurons were observed by histopathology and transmission electron microscopy,the expression of PGC-1α,TFAM,Bcl-2,Bax,Caspas-3 factors in hippocampus at mRNA and protein levels was detected by RT-PCR and Western blotting methods,and the apoptosis rate of hippocampal neurons was detected by TUNEL.The structure of hippocampus cells in the acute hypoxia model group was disordered in different degrees on the 1st,3rd,5th and 7th day,while that in the crocin group was obviously improved.The mRNA and protein level of PGC-1α,TFAM,Bcl-2 in hippocampus of rats was higher in treatment group than that of in model group,and the expression level in 1 d,3 d,5 d were obviously higher(p<0.05),there was no significant differences at 7 d in two groups(p>0.05).Western blotting and RT-PCR showed that the protein and mRNA expression level of Bax,Caspase-3 in hippocampus of rats was lower in treatment group than that of the model group,and the expression levels at 1 d,3 d,5 d and 7 d were obviously lower(p<0.05),there was no significant differences at 7 d in two groups(p>0.05).Compared with the model group,treatment group showed significantly lower in the apoptosis rate at 1 d,3 d,5 d(p<0.05).Crocin could ameliorate mitochondrial damage and reduce the apoptosis in hippocampus at acute high altitude hypoxia conditions.This research will be helpful for pharmacological prevention of high altitude diseases.
作者
张晓岩
张先钧
蒲小燕
王海燕
李占强
芦殿香
Zhang Xiaoyan;Zhang Xianjun;Pu Xiaoyan;Wang Haiyan;Li Zhanqiang;Lu Dianxiang(Research Center for High Altitude Medicine,Qinghai University,Xining,810001;Department of Basic Medicine,Qinghai University Medical Col lege,Xining,810001;Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province(Qinghai-Utah Joint Research key Lab for High Altitude Medicine),Xining,810001)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第4期1722-1731,共10页
Genomics and Applied Biology
基金
国家自然科学基金项目(81260685)
青海省科技厅基础研究项目(2016-ZJ-708)共同资助。