miR-132基因沉默改善PTSD大鼠焦虑样行为与上调FXR1表达相关

miR-132 ameliorates anxiety-like behavior via upregulating fragile X-related gene 1 in PTSD rats

目的研究创伤后应激障碍(PTSD)大鼠前额叶皮质脆性X染色体相关基因1(FXR1)的表达变化,探讨miR-132基因沉默对PTSD大鼠焦虑样行为的影响。方法采用单程长时应激(SPS)制备PTSD大鼠模型,通过侧脑室注射慢病毒建立miR-132基因沉默的PTSD大鼠。将大鼠分为4组:正常组、PTSD组、sh-miRNC组和sh-miR组,通过旷场实验和高架十字迷宫实验检测miR-132基因沉默对PTSD大鼠焦虑样行为的影响。取材各组大鼠前额叶皮质,利用免疫荧光染色、Western blot检测FXR1蛋白表达情况。结果与正常对照组大鼠相比,PTSD组大鼠有显著的焦虑样行为,miR-132基因沉默显著减轻大鼠的焦虑样行为。与正常对照组大鼠相比,PTSD组大鼠前额叶皮质FXR1蛋白表达水平显著降低,而miR-132基因沉默显著上调FXR1蛋白表达水平。结论miR-132基因沉默显著减轻PTSD大鼠的焦虑样行为与上调FXR1表达水平相关。

Objective To investigate the expression of fragile X-related gene 1(FXR1)in the prefrontal cortex of rats with post-traumatic stress disorder(PTSD),and to explore the effect of microRNA-132(miR-132)gene silence on anxiety-like behavior via regulating FXR1 in PTSD rats.Methods The PTSD model was established by single prolonged stress paradigm(SPS)and microRNA-132 gene was silenced by injecting lentivirus into the lateral ventricle of PTSD rats.The rats were divided into four groups:Control,PTSD,sh-miR-NC,and sh-miR.Open-field test and elevated plus-maze test were used to detect the anxiety behavior of rats.The expression of FXR1 protein in PFC was detected by immunocytochemistry and Western blot.Results miR-132 gene silencing ameliorates anxiety symptoms of PTSD rats significantly.The expression level of FXR1 in prefrontal cortex was decreased significantly in PTSD rats than in normal control rats,but increased significantly in miR-132 gene silencing rats than in PTSD rats.Conclusion miR-132 gene silence ameliorates anxiety-like behavior via upregulating fragile X-related gene 1 in PTSD rats.