摘要
目的探究外源性趋化素对卵巢癌细胞生物学特性的影响。方法体外培养卵巢癌细胞HO-8910,利用免疫印迹法检测HO-8910与HO-8910PM细胞株中Chemerin含量,给予外源性的Chemerin蛋白处理后,利用WST分析外源性的Chemerin蛋白对卵巢癌细胞增殖能力的影响,利用划痕实验观察外源性的Chemerin蛋白对卵巢癌细胞迁移能力的影响,利用PI染色观察外源性Chemerin蛋白对卵巢癌细胞的细胞周期的影响。结果Chemerin蛋白在HO-8910细胞株中表达水平明显低于HO-8910PM细胞株,外源性Chemerin的重组蛋白对卵巢癌细胞的细胞周期进程整体无明显影响,因此并不影响其增殖的速率,但可以促进卵巢癌细胞的迁移。结论外源性的Chemerin作为脂肪因子发挥的是促细胞迁移的效应,在一定程度上揭示了来源于腹腔脂肪细胞的脂肪因子在卵巢癌腹腔转移过程中可能发挥了趋化作用或促迁移的作用。
Objective To explore the effect of exogenous Chemerin on the biological characteristics of ovarian cancer cells.Methods HO-8910 cells were cultured in vitro.Western blot was used to analyze Chemerin in HO-8910 and HO-8910 PM cell lines,HO-8910 cells were treated with exogenous Chemerin.WST was used to analyze the effect of exogenous Chemerin on the proliferation of ovarian cancer cells.Scratch test was used to observe the effect of exogenous Chemerin on the migration of ovarian cancer cells.Effect of exogenous Chemerin protein on cell cycle of ovarian cancer cells was observed by PI staining.Results The expression level of Chemerin in HO-8910 cell line was significantly lower than that in HO-8910 PM cell line.The recombinant protein of exogenous Chemerin did not affect the cell cycle process of ovarian cancer cells,so it did not affect the proliferation rate,but could promote the migration of ovarian cancer cells.Conclusion As an adipokine,exogenous Chemerin plays a role in promoting cell migration.To a certain extent,it reveals that adipokine derived from peritoneal adipocytes might play a role in chemotaxis or promoting migration in the process of peritoneal metastasis of ovarian cancer.
作者
李奇
高晨曦
芦恩婷
张颐
LI Qi;GAO Chen-xi;LU En-ting;ZHANG Yi(Department of Gynecology,the First Affiliated Hospital,China Medical University,Shenyang 110001,China)
出处
《解剖科学进展》
2020年第3期318-320,324,共4页
Progress of Anatomical Sciences