一种小鼠无创气管插管的方法及其应用

A simple method and the application of mouse tracheal intubation

无创气管插管是临床常用技术手段,但对实验小鼠进行无创气管插管具有一定难度。特别是对同一小鼠进行反复气管插管极易对小鼠咽喉部造成损伤。本研究提供了一种便捷可靠的小鼠气管插管方法,可针对同一实验动物进行反复无创操作,进而可用于各类药理学及其他实验。本实验所有动物实验均通过中国医学科学院药物研究所伦理审查委员会审查。应用此种方法同时配合小鼠肺功能检测仪,本课题组绘制了小鼠肺功能发育曲线,发现小鼠在8周龄或18~20 g时肺功能发育成熟。同时应用此方法,本课题组成功制备了多次博莱霉素所致慢性肺纤维化模型。因此,该小鼠无创气管插管方法是安全可靠的药理学实验手段。

Although noninvasive trachea cannula is a common technique in clinic,this technique is hard to be applied in mice.It is even more difficult to perform the repeated noninvasive trachea cannula on the same mouse due to the throat injury.Here we report an effective,rapid and easy method to perform the noninvasive intubation on mice.This method can effectively be applied to many pharmacological and other related studies.In addition,this strategy can protect the mouse from physical injury caused by operative procedure.All procedures involving animal treatment were approved according to the Committee on the Ethics of Animal Experiments of the Institute of Materia Medica,Chinese Academy of Medical Sciences.In alliance with lung function measurement system,we detected the normal lung function values and presented the lung function development curves of mice of different age.We found that the lung function of mice was matured at 8 weeks old or at the body weight of 18-20 g.In addition,we constructed the mouse model of multiple bleomycin induced pulmonary fibrosis by using this method and illustrated that the trachea cannula is more efficiency than nasal inhalation.Using this method,we have confirmed that the immune microenvironment in lung tissue of multiple bleomycin model is distinct from that of single bleomycin induced pulmonary fibrosis model.Thus,this method is a reliable and safe strategy for the pharmacology study.