绿豆皮中黄酮树脂纯化条件优化及纯化物分析

Optimization of Purification Condition and Purification Material Analysis of Flavone Resin in Mung Bean Skin

以明绿豆黄酮提取液为试验材料,AB-8型树脂吸附量为指标,通过考查不同样液质量浓度、流速、pH值、径高比等单因素,确定树脂吸附效果,通过正交试验对大孔树脂的动态吸附条件优化获得最优的吸附条件组合,并通过红外光谱和扫描电镜对纯化物进行定性鉴定和微观结构分析。结果表明,样液质量浓度是影响绿豆黄酮纯化的效果的显著因素,其他因素影响依次为径高比、流速、pH值,优化后的树脂吸附条件中的最佳样液质量浓度为1 mg/mL,最佳流速为2 mL/min,最佳pH值为5,最佳径高比为1∶15,优化后树脂吸附量达到24.38 mg/g,较优化纯度前提高44.03%,单因素试验筛选出最佳解析条件为体积分数为95%,用量为175 mL。

Ming mung bean extract flavonoids as test materials,AB-8 resin adsorption quantity as examining index,by investigating the liquid with different mass concentration,flow velocity,pH,diameter ratio,such as single factor,determine the resin adsorption effect,through the orthogonal experiment conditions of macroporous resin adsorption dynamic optimization to obtain the optimal combination of adsorption conditions,and through infrared spectrum and scanning electron microscopy(sem)of the purification of qualitative identification and microstructure analysis.The results showed that the sample liquid mass concentration were significant factors affecting the effects of mung bean purified flavonoid,other factors affect the ratio of diameter,flow velocity,pH value,optimized the best sample liquid resin adsorption conditions,mass concentration of 1 mg/mL,the optimal flow rate of 2 mL/min,the optimum pH for 5,best diameter ratio was 1∶15,optimized resin adsorption reached 24.38 mg/g,the optimization of purity before increased by 44.03%,the single factor selected the optimal analytical conditions for the liquid integral number was 95%,adding amount of 175 mL.