子宫肌瘤与雌激素代谢酶CYP1A1和COMT基因多态性的关系

The Relationship between Uterine Leiomyoma and the Polymorphisms of Estrogen Metabolizing Enzymes CYP1A1 and COMT Genes

目的探究子宫肌瘤与雌激素代谢酶细胞色素酶450A1和儿茶酚氧位甲基转移酶基因多态性的关系。方法随机选取某院2016年1月2日-2017年12月31日收治的子宫肌瘤患者89例作为观察组,另选取同期健康体检者100例作为对照组,2组开展聚合酶链反应-限制性片段长度多态性分析,对CYP1A1和COMT基因进行测定,分析上述两者基因与子宫肌瘤相关性。结果 2组基因型分布均符合Hardy-Weinberg遗传平衡定律,观察组CYP1A1基因型频率TT、TC、CC等位基因频率T、C占比均低于对照组,差异有统计学意义(P<0.05),2组基因型分布均符合Hardy-Weinberg遗传平衡定律,观察组COMT基因型频率GG、GA、AA、等位基因频率G、A占比均低于对照组,差异有统计学意义(P<0.05)。结论子宫肌瘤发病与CYP1A1基因Mspl位点多态性以及COMT基因Val158Met多态性可能存在相关性。

Objective To investigate the relationship between the uterine leiomyoma and the polymorphisms of estrogen metabolizing enzymes CYP1 A1 and catechol-O-methyltransferase(COMT)genes.Methods 89 patients with uterine leiomyoma from January,2016 to December,2017 in a hospital were randomly selected as observation group,and 100 healthy people from the same period were selected as control group.The two groups performed PCR-restriction fragment length polymorphism analysis,CYP1 A1 and COMT genes were determined to analyze the correlation between these two genes and uterine fibroids.Results The genotype distribution of the two groups was in accordance with Hardy Weinberg’s law of genetic balance,the genotype frequency of TT,TC,CC,allele T and C of CYP1 A1 genotypes of the observation group were lower than those of the control group,the difference between the observation group and the control group was statistically significant(P<0.05),the genotype distribution of the two groups was in accordance with Hardy Weinberg’s law of genetic balance,the proportion of COMT genotype frequency GG,GA,AA,allele frequency G and A of the observation group was lower than those of the control group,and the difference was statistically significant(P<0.05).Conclusions There may be correlation between the occurrence of uterine leiomyoma and Mspl polymorphism of CYP1 A1 gene and Val158 Met polymorphism of COMT gene.