咸宁城区被动吸烟人群尿中可替宁和3-羟基可替宁的检测

Detection of urinary cotinine and 3-hydroxycotinine of passive smokers in Xianning urban area

目的探讨咸宁城区被动吸烟人群被动吸烟量与尿中可替宁、3-羟基可替宁浓度的关系,准确评价被动吸烟人群烟草暴露水平,为政府制定控烟政策提供科学依据。方法根据被动吸烟指数等级分为3组,1~9、10~19和≥20支/天,采集咸宁城区被动吸烟人群尿样共120份(每组40份),另外,采集咸宁市疾病预防控制中心工作人员中本人不吸烟,且最近3个月无烟草暴露史的40人作为空白对照,对其工作生活环境等情况进行问卷调查。利用气相色谱-质谱检测法测定尿中2种尼古丁的代谢产物可替宁、3-羟基可替宁的含量,并对结果进行统计分析。结果被动吸烟量与尿液中的可替宁和3-羟基可替宁浓度均呈正相关(r=0.872、0.745,均P<0.01)。随着被动吸烟量的增加,尿液中可替宁、3-羟基可替宁含量均升高。结论被动吸烟可以增加尿液中可替宁、3-羟基可替宁的含量,可替宁和3-羟基可替宁可以作为反映被动吸烟人群烟草暴露水平较好的标志物。

Objective To explore the relationship between the amount of passive smoking and the concentrations of cotinine and 3-hydroxycotinine in urine of passive smokers in Xianning urban area,evaluate the tobacco exposure levels of passive smokers,and provide scientific basis for the government to formulate tobacco control policies.Methods A total of 120 urine samples(40 in each group)were collected from passive smokers in Xianning City,and they were divided into three groups according to the level of passive smoking index:1-9,10-19 and≥20 cigarettes per day.In addition,40 people who were non-smokers and had no recent tobacco exposure in Xianning Center for Disease Control and Prevention were randomly collected as the blank control group,to conduct the questionnaire survey on their working and living environment.The contents of cotinine and 3-hydroxycotinine in urine were determined by gas chromatography-mass spectrometry,and the results were analyzed statistically.Results The amount of passive smoking was positively correlated with the contents of cotinine and 3-hydroxycotinine in urine(r=0.872,0.745,both P<0.01).The contents of cotinine and 3-hydroxycotinine in urine increased with the increase of the amount of passive smoking.Conclusion Passive smoking can increase the contents of the cotinine and 3-hydroxycotinine in urine,and cotinine and 3-hydroxycotyl can be used as the better markers to reflect the tobacco exposure level of passive smokers.