基于顶空固相微萃取气相质谱联用技术分析当归川芎药对配伍前后挥发性组分变化规律

Analysis of Volatilis Components Changes Before and After Combination in Angelica and Ligusticum by Headspace Solid Phase Microextraction Gas Chromatography-Mass Spectrometry

目的分析比较全当归、川芎、全当归-川芎药对以及当归尾-川芎药对的挥发性成分。方法通过单因素考察确定最佳萃取条件,采用顶空固相微萃取法(HS-SPME)与气相色谱-质谱(GC-MS)联用,测定当归、川芎、全当归-川芎药对以及当归尾-川芎药对挥发性化学成分,通过面积归一法确定各成分含量。结果最终确定条件为称量量0.5 g,萃取温度80℃,萃取时间15 min,解析附时间3 min。全当归药材粉末定性化学成分34个;川芎药材粉末定性化学成分51个;全当归-川芎药材粉末定性化学成分47个;当归尾-川芎药材粉末定性化学成分39个。结论:当归和川芎的主要挥发性成分为藁本内酯和洋川芎内酯。

Objective To analyze and compare the volatile components of Danggui(Angelica),Chuanxiong(Ligusticum)and couplet medicines.Methods The optimal extraction conditions were determined by single factor test.Headspace solid-phase microextraction(HS-SPME)was used in combination with gas chromatography-mass spectrometry(GC-MS)to determine the medicinal parts volatile chemicals of Danggui(Angelica),Chuanxiong(Ligusticum)and couplet medicines,determined the content of each component by area normalization.Results The optimal extraction conditions were weighing 0.5 g,extraction temperature 80℃,extraction time 15 min,analysis time 3 min.There were 34 qualitative chemical componentsof tail parts of Danggui(Angelica)and 51 qualitative chemical componentsof Chuanxiong(Ligusticum).The Danggui(Angelica)-Chuanxiong(Ligusticum)couplet medicines had 47 qualitative chemical components.Conclusion The main volatile components of Danggui(Angelica)and Chuanxiong(Ligusticum)are ligustilide and senkyunolide.