健脾养胃方防治胃癌的网络药理学机制研究

Study on Network Pharmacological Mechanism of Jianpi Yangwei Recipe in Prevention and Treatment of Gastric Cancer

目的基于网络药理学技术研究健脾养胃方防治胃癌的作用机制。方法从相关数据库中收集并筛选有效化学成分和有效靶点。采用Cytoscape软件构建有效化学成分-靶点网络,结合CytoNCA插件完成拓扑分析。通过String 10.0软件建立蛋白相互作用(PPI)网络,结合Centiscape 2.2插件获得核心蛋白。经DAVID平台和ClueGO插件完成GO分析和KEGG分析。结果筛选出145个有效化学成分,有效靶点中药物靶点占151个、胃癌靶点占911个、共同靶点占88个。有88个蛋白质节点参与PPI网络,其中核心蛋白涉及15个。关键靶点不仅参与调控胃癌的细胞转录、MAPK激活、缺氧应激、细胞凋亡、周期和衰老等过程,还介导NOD-Like受体信号通路、PI3K-Akt信号通路、HIF-1信号通路、VEGF信号通路等。结论通过挖掘中药复方作用于胃癌的潜在靶点和有关通路,推测健脾养胃方可能具有防治胃癌的效用,为中医现代化发展奠定坚实的基础。

Objective To study the mechanism of Jianpi Yangwei Recipe in the prevention and treatment of gastric cancer based on network pharmacology.Methods The effective chemical constituents and effective targets were collected and screened from the relevant databases.Cytoscape software was used to construct the effective chemical composition-target network,and CytoNCA plug-in was used to complete the topology analysis.The PPI network of protein interaction was established by String10.0 software,and the core protein was obtained by combining with Centiscape 2.2 plug-in.GO analysis and KEGG analysis were completed by DAVID platform and ClueGO plug-in.Results A total of 145 effective chemical constituents were screened out,of which 151 were drug targets,911 were gastric cancer targets and 88 were common targets.There were 88 protein nodes involved in the PPI network,of which 15 core proteins were involved.The key targets were not only involved in the regulation of cell transcription,MAPK activation,hypoxia stress,apoptosis,cycle and aging of gastric cancer,but also mediated NOD-like receptor signaling pathway,PI3 K-Akt signaling pathway,HIF-1 signaling pathway,VEGF signaling pathway and so on.Conclusion By excavating the potential targets and related pathways of traditional Chinese medicine compound prescription on gastric cancer,it is speculated that Jianpi Yangwei Recipe may have the effect of preventing and treating gastric cancer,which lays a solid foundation for the development of modernization of traditional Chinese medicine.