酸碱度和松树基质对杀线菌Esteya vermicola菌丝体生长的影响

Effects of pH Value and Pine Substrate on Growth of Esteya vermicola Mycelia

[目的]研究Esteya vermicola真菌适应的pH范围,并探讨松树成分对其生长的影响,以期为E.vermicola菌在松树体内的定植和林业重大病害松材线虫野外的生物防治提供理论支撑。[方法]选取E.vermicola CBS115803(EV115803)和E.vermicola CNU120806(EV120806)为试验对象,测试EV真菌适应的pH范围,并以35 a黑松不同器官组织配制培养基,探究EV菌的生长情况,观测各菌株生长速率、菌体量和产孢量。[结果]EV菌适应的pH值范围为5~13,菌体量在酸性条件下产生更多的气生菌丝。PDA中加入松树成分对EV菌有一定的刺激生长作用。在以松针、松树皮、松枝条为唯一营养来源时,EV菌生长茂盛。以木质部锯末为单一营养源时,EV菌生长缓慢。[结论]EV菌适应的pH值范围为5~13,EV菌耐碱性极强,且在加有松树各部位成分的培养基上生长,菌株EV120806对松树培养基的适应性更强,未来有望将EV菌定殖在松树中做为共生菌,摄食入侵的松材线虫,从而使松树具有内源的防控因子防控松材线虫。

[Objective]To study the pH range favoring Esteya vermicola,and the effects of pine substrate on the growth of E.vermicola so as to provide references for colonization of E.vermicola in Pinus trees and the biological control against pine wood nematodes.[Method]E.vermicola CBS115803(EV115803)and E.vermicola CNU120806(EV120806)were used to test their fungal pH adaptation range.The media with different parts of pine were prepared to study the growth of E.vermicola and to observe and measure the growth rate,mycelial amount and sporulation of each strain.[Result]E.vermicola was adapted to the pH range of 5 to 13,and the fungi produced more aerial hyphae under acidic conditions.PDA medium added with different parts of pine trees had a certain stimulating effect for E.vermicola.When pine needles,pine bark,and pine twigs were used as the only nutrient source,E.vermicola grew better than on WA plate.E.vermicola strains grew slowly when xylem sawdust was used as a single nutrient source.[Conclusion]E.vermicola is adapted to the pH range of 5 to 13.It is extremely resistant to alkaline and can grow on media added with different parts of pine trees.It is expected that E.vermicola will colonize the pine trees as symbiotic fungi against invading pine wood nematodes,so that pine trees have endogenous control factors for pine wood nematodes.