摘要
[目的]对野生食用菌进行分子鉴定及生物学特性研究,为其进一步液体深层发酵培养和开发利用奠定基础。[方法]采用rDNA ITS序列分析方法对一野生菌进行分子鉴定,通过单因素和正交试验确定了该菌株的生物学特性。[结果]由新鲜子实体分离得到的纯菌株为Paxillus ammoniavirescens,其菌丝体生长的最适碳源和氮源分别为可溶性淀粉和蛋白胨,最适培养温度为25℃,最适pH为4.5。[结论]卷边网褶菌菌丝体液体培养的最佳配方为:可溶性淀粉20 g,蛋白胨2 g,KH2PO43.0 g,MgSO44.5 g,水1 L。按此配方培养,可获得平均干质量为80 mg的菌丝体,明显高于其他配方。
[Objective]To Understand the molecular identity and biological characteristics of wild edible fungi and lay foundation for their further culture,development and utilization.[Method]A wild strain of edible mushroom was examined by rDNA ITS sequence analysis and its biological characteristics were investigated by single factor and orthogonal test.[Result]The molecular feature of the mycelia generated from the fresh fruit body of the edible mushroom was proved to be Paxillus ammoniavirescens.The optimal carbon and nitrogen sources of its mycelial growth were found to be soluble starch and peptone with most favorable conditions of 25℃and pH 4.5.[Conclusion]The best formula for mycelial liquid culture is 20 g soluble starch,2 g peptone,3.0 g KH2PO4,4.5 g MgSO4 and 1 liter of water.In average,80 mg dry weight of mycelia can be generated,which is obviously higher than other culture formulas.
作者
杜萍
凃慧
蒋佳颖
崔宝凯
戴玉成
DU Ping;TU Hui;JIANG Jia-ying;CUI Bao-kai;DAI Yu-cheng(School of Advanced Agriculture and Bioengineering,Yangtze Normal University,Chongqing 408100,China;Institute of Microbiology,Beijing Forestry University,Beijing 100083,China)
出处
《林业科学研究》
CSCD
北大核心
2020年第3期146-155,共10页
Forest Research
基金
国家自然科学基金项目(31900019)
重庆市教育委员会项目(KJQN201901427)
长江师范学院2018年人才引进项目(0107/011160022)。