Linc01635对川崎病血管内皮细胞凋亡的影响

The effect of linc01635 on apoptosis of vascular endothelial cells in Kawasaki disease

目的:探讨linc01635对川崎病(KD)血管内皮细胞凋亡的影响。方法:通过生物信息学网站预测和RNA FISH检测来分析linc01635的亚细胞定位。用KD患者血浆或健康儿童血浆刺激人脐静脉内皮细胞(HUVECs),qRT-PCR检测HUVECs内linc01635的相对表达量。通过慢病毒过表达或siRNA敲减HUVECs中的linc01635,流式细胞术分析细胞凋亡情况。用KD血浆诱导细胞凋亡,检测linc01635在KD诱导血管损伤中的作用。结果:Linc01635在HUVECs中主要分布于细胞核和细胞质。与健康对照相比,KD血浆培养的HUVECs中的linc01635相对表达量下降(P<0.01)。Linc01635过表达抑制了HUVECs的凋亡(P<0.01),而linc01635敲减使HUVECs凋亡增加(P<0.01)。KD血浆能促进HUVECs凋亡,而过表达linc01635能抑制KD血浆诱导的凋亡(P<0.01)。结论:KD血浆处理会导致血管内皮细胞中linc01635表达量下降,而linc01635的减少会促进血管内皮细胞凋亡。

Objective:To investigate the effect of linc01635 on apoptosis of vascular endothelial cells in Kawasaki disease(KD).Methods:Bioinformatics prediction and RNA FISH was employed to detect Subcellular localization of linc01635.Human umbilical vein endothelial cells(HUVECs)were cultured using KD plasma and healthy control plasma,qRT-PCR was used to detect the relative expression of linc01635 in HUVECs.Linc01635 expression in HUVECs was knocked-down by linc01635 siRNA and up-regulated by lentivirusor.Flow cytometry was used to analyze the apoptosis of HUVECs.Apoptosis was induced by KD plasma and the effect of linc01635 on KD-induced vascular endothelial cell injury was detected.Results:Linc01635 was distributed in both nuclei and cytoplasm of HUVECs.Compared with the healthy control group,KD plasma-treated HUVECs exhibited reduced expression of linc01635(P<0.01).Overexpression of linc01635 inhibited HUVECs apoptosis(P<0.01),while knockdown of linc01635 increased HUVECs apoptosis(P<0.01).KD plasma could promote HUVECs apoptosis,and overexpression of linc01635 could inhibit KD plasma-induced apoptosis(P<0.01).Conclusion:KD plasma treatment can reduce the expression of linc01635 in vascular endothelial cells,which decreased linc01635 via KD plasma stimulation can induce vascular endothelial cells apoptosis.