聚多巴胺涂层的伪模板分子印迹聚合物用于分离白头翁皂苷B4

Polydopamine-coated magnetic dummy molecularly imprintedpolymers for separation of Anemoside B4

以四氧化三铁(Fe 3O 4)为支撑材料、鼠李糖(Rha)为模板分子、盐酸·多巴胺(DA·HCl)为功能单体,成功合成磁性表面分子印迹聚合物(Rha-MMIPs),并将其用于白头翁中白头翁皂苷B4(AB4)预富集研究。利用扫描电镜(SEM)、透射电镜(TEM)、红外光谱(FTIR)、热重分析(TGA)、磁性分析等手段对Rha-MMIPs进行表征,并对其吸附性能及重现性进行考察。结果表明,Rha-MMIPs为壳-核球型结构,热稳定性好,具有良好的吸附性能(5.79 mg/g)和快速吸附能力(60 min),对AB4的动态吸附符合准二级动力学模型,吸附过程为Langmuir单层吸附,重现性良好。将Rha-MMIPs用于白头翁中AB4的预富集,采用高效液相色谱仪(HPLC)进行检测,结果显示该聚合物可用于从复杂样品中分离富集AB4。

The magnetic molecularly imprinted polymers(Rha-MMIPs)were successfully prepared using Fe 3O 4as carrier material,Rha as dummy template and dopamine(DA·HCl)as the functional monomer.The Rha-MMIPs were applied to study the Anemoside B4(AB4)of Pulsatilla chinensis(Bge.)Regel.The resultant polymers were characterized by SEM,TEM,FT-IR and TGA.The adsorption properties were also investigated.The results showed that Rha-MMIPs exhibited excellent adsorption performance,good adsorption capacity(5.79 mg/g),fast kinetics(60 min)and thermal stability.It was obvious the pseudo-second-order model fitted the experimental data better.And the results showed that the synthesized Rha-MMIPs showed monolayer adsorption behavior.Furthermore,Rha-MMIPs were employed as adsorbents for extraction and enrichment of AB4 from Pulsatilla chinensis(Bge.)Regel extract.The results proved that the polymers have the potential to provide a selective recognition of AB4 in complex samples.