巨噬细胞特异性敲除TSC1小鼠的构建

Construction of macrophage-specific knockout TSC1 mice

目的为研究TSC1在巨噬细胞极化中的作用,拟建立巨噬细胞特异性敲除TSC1小鼠,提供稳定的动物模型。方法利用Cre-loxP系统构建巨噬细胞特异性敲除TSC1小鼠(LysM-Cre TSC1flox/flox),并通过PCR和Western-Blot鉴定模型建立是否成功。结果通过PCR和Western-Blot鉴定LysM-Cre TSC1flox/flox模型建立成功,mTOR活性明显升高。结论成功构建巨噬细胞特异性敲除TSC1小鼠,为TSC1在巨噬细胞极化中的作用研究提供实验基础。

Objective To study the role of TSC1 in macrophage polarization in order to establish a macrophage-specific knockout TSC1 mice and to provide a stable animal model.Methods The Cre-loxP system was used to construct a macrophage-specific knockout TSC1 mice(LysM-Cre TSC1flox/flox),and the model was established by PCR and Western-Blot.Results The LysM-Cre TSC1flox/flox model was established by PCR and western-blot successfully,and the mTOR activity was significantly increased in the model.Conclusion The macrophage-specific knockout TSC1 mice is successfully constructed and it can provide an experimental basis for the role of TSC1 in macrophage polarization.