用于汞离子检测的近红外荧光分子探针合成与评价

Synthesis and evaluation of a molecular near-infrared fluorescent probe for detecting mercury

设计合成了一种可特异性识别Hg^2+的小分子近红外荧光探针P-22,并完成对其体内、外光学响应性能的评价.该探针可在水溶液中被Hg2+催化发生烯醚的水解反应,从而释放出一种具有近红外荧光性质的花菁类染料qCy7,导致反应体系的最大发射波长由560 nm红移至705 nm,通过检测反应体系中荧光强度的变化实现对Hg^2+的半定量分析.实验结果证明,本研究所设计合成的近红外荧光探针P-22能够同时实现对体外水环境中和复杂生物体环境中的Hg2+进行高选择性、高灵敏度地实时检测,为进一步研究有害金属汞在自然环境及复杂的生物体内的作用提供了有力的工具.

A molecular near infrared fluorescent probe P-22 that can specifically recognize Hg2+was designed and synthesized in this study,and its optical characteristics towards mercury was evaluated both in vivo and in vitro.The probe P-22 with a vinyl ether moiety could be selectively hydrolyzed by Hg2+to generate a Cyanine-derived near-infrared dyes(qCy7),resulting in a red shift of the maximum emission wavelength of the system from 560 nm to 705 nm.Thus,the amount of Hg2+could be semi-quantitatively detected through the change of the fluorescence intensity.The results of our study demonstrate that the fluorescent probe P-22 we developed has a capability for the detection of mercury with superior selectivity and high sensitivity both in aqueous environment and complex biological system.It provides a powerful tool to further study the role of the notoriously poisonous mercury in the natural environment and complex organisms.