YEATS2在肝细胞癌中的表达及临床意义

Expression of YEATS2 in hepatocellular carcinoma and its clinical significance

目的 探讨YEATS2在肝细胞癌(hepatocellular carcinoma,HCC)中的表达及其与预后的关系.方法 收集我院2010年1月至2014年1月1 12例手术切除的HCC癌组织和癌旁组织石蜡标本,采用Real-time PCR检测HCC癌组织及癌旁组织中YEATS2 mRNA的表达,Western blot和免疫组化染色检测YEATS2蛋白水平,免疫荧光染色检测YEATS2在HCC癌组织中的表达定位,并分析YEATS2与HCC患者临床病理特征和预后的关系,同时采用TCGA数据库数据验证.结果 Real-time PCR结果显示,YEATS2 mRNA在HCC癌组织中的表达较癌旁组织上调(P<0.001);Western blot检测结果显示,YEATS2在约66.67%(12/18)的HCC癌组织表达升高;免疫组化染色分析发现,约71.43%(80/112)的HCC患者中YEATS2表达上调,其表达与TNM分期明显相关(P<0.001);YEATS2主要在HCC癌组织细胞核中表达;多因素分析结果显示YEATS2高表达是患者不良预后的独立危险因素(HR=2.204,95%CI:1.388~3.500,P=0.001).TCGA中HCC队列分析也表明YEATS2高表达的患者OS更差(HR=1.751,95%CI:1.107~2.770,P=0.017).结论 YEATS2在HCC组织中高表达,且与患者不良预后相关.

Objective To investigate the expression of YEATS Domain Containing 2(YEATS2)in hepatocellular carcinoma(HCC) and its relationship with prognosis. Methods A total of 112 cases of paraffin specimens of liver cancer and adjacent tissues surgically removed in our hospital from January 2010 to January 2014 were collected. Real-time PCR was used to determine the expression of YEATS2 mRNA in HCC tissues and adjacent tissues,Western blot and Immunohistochemical staining were used to detect the protein level of YEATS2,Immunofluorescence staining was used to detect the localization of YEATS2 in HCC tissues and the correlation between YEATS2 and clinicopathological characteristics and prognosis of HCC patients were analyzed,the results were verified by TCGA database. Results Real-time PCR results showed that the expression of YEATS2 mRNA in HCC tissues was up-regulated more significantly than that in adjacent tissues(P<0.001). Western blot showed that the expression of YEATS2 was increased in approximately 66.67%(12/18)HCC tissues. Immunohistochemical staining founded that YEATS2 expression was up-regulated in 71.43%(80/112)HCC patients,and its expression was significantly correlated with TNM stage(P<0.001). YEATS2 was mainly expressed in nucleus of hepatocellular carcinoma. Multivariable analysis showed that high expression of YEATS2 was an independent risk factor for poor prognosis of HCC patients(HR=2.204,95%CI:1.388-3.500,P=0.001). The HCC cohort analysis in TCGA also showed that patients with high expression of YEATS2 had poorer OS(HR=1.751,95%CI:1.107-2.770,P=0.017). Conclusion YEATS2 is highly expressed in HCC tissues and correlated with poor prognosis of HCC patients.