鲫鱼皮胶原蛋白理化性质及自组装行为研究

Physicochemical properties and self-assembly behavior of collagen from Carassiusauratus skin

以鲫鱼皮为原料,采用酸法和酶法提取鲫鱼皮中的胶原蛋白,研究鲫鱼皮酸溶性和酶溶性胶原蛋白的理化性质及自组装行为.结果表明,鲫鱼皮酸溶性和酶溶性胶原蛋白的氨基酸组成基本一致,均含有丰富的甘氨酸,含量高达32.65%左右.鲫鱼皮酸溶性和酶溶性胶原蛋白由2条不同的α肽链和1条β肽链组成,是典型的Ⅰ型胶原蛋白.鲫鱼皮酸溶性和酶溶性胶原蛋白在波长218 nm处具有较强的紫外吸收,其二级结构中β-折叠含量最高,而α-螺旋含量则最低.自组装行为研究结果表明,胶原蛋白浓度、pH值、NaCl浓度和温度对鲫鱼皮酸溶性和酶溶性胶原蛋白的自组装行为均有明显影响,在胶原蛋白浓度2.1 mg/mL、pH 7.2、NaCl浓度200~600 mmol/L和温度20℃的条件下,鲫鱼皮酸溶性胶原蛋白的自组装程度较好;在胶原蛋白浓度1.3 mg/mL、pH 7.2、NaCl浓度200~400 mmol/L和温度20℃的条件下,鲫鱼皮酶溶性胶原蛋白的自组装程度较好.

The fish collagen was extracted from Carassiusauratus skin by acid-soluble method and enzyme hydrolysis,respectively.The obtained acid-soluble collagen(ASC)and pepsin-soluble collagen(PSC)were used to study their physicochemical properties and self-assembly behavior.The results showed that the amino acid compositions of ASC and PSC were basically the same.ASC and PSC are rich in glycine,and their glycine content is as high as about 32.65%.The ASC and PSC from Carassiusauratus skin were characterized as a typeⅠcollagen with two differentα-chains and oneβ-chain and had strong ultraviolet absorption at the wavelength of 218 nm.Their secondary structure has the highestβ-sheet content and the lowestα-helix content.The self-assembly behavior of ASC and PSC from Carassiusauratus skin could be influenced by collagen concentration,pH,NaCl concentration and temperature.The ASC from Carassiusauratus skin had a good self-assembly degree at collagen concentration of 2.1 mg/mL,pH of 7.2,NaCl concentration of 200~600 mmol/L and temperature of 20℃,while the PSC from Carassiusauratus skin had a good self-assembly degree at collagen concentration of 1.3 mg/mL,pH of 7.2,NaCl concentration of 200~400 mmol/L and temperature of 20℃.