摘要
目的研究褪黑素对人骨肉瘤MG63、U2细胞p53、c-Myc基因表达的调控。方法体外培养MG63、U2细胞,4mmol·L^-1褪黑素干预24h,实时荧光定量PCR及免疫印迹法检测p53及c-Myc基因表达情况。结果褪黑素作用后,U2细胞上调p53基因表达(P<0.05);MG63、U2细胞下调c-Myc蛋白水平表达(P<0.05)。结论褪黑素可能通过上调p53基因及下调c-Myc蛋白表达促进骨肉瘤细胞凋亡。
OBJECTIVE To research the regulation of melatonin on the expression of p53 and c-Myc genes in human osteosarcoma MG63 and U2 cells.METHODS MG63 and U2 cells were cultured in vitro and treated with 4mmol·L-1 melatonin for 24h.The expression of p53 and c-Myc gene were detected by real-time fluorescence quantitative PCR and western blot.RESULTS After the treatment of melatonin the expression of p53 gene in U2 cells increased significantly(P<0.05)and the expression of c-Myc protein in MG63 and U2 cells significantly decreased(P<0.05).CONCLUSION Melatonin may promote the apoptosis of osteosarcoma cells by up-regulating the expression of p53 gene and down-regulating the expression of c-Myc protein.
作者
林晋
唐风华
黄铭珊
许颖
黄志鸿
LIN Jin;TANG Feng-hua;HUANG Ming-shan;XU Ying;HUANG Zhi-hong(Department of Basic Subjects,Fujian Health College,Fuzhou 350101,China;Public technology service center,Fujian Meidcal University,Fuzhou 350122,China)
出处
《海峡药学》
2020年第6期6-9,共4页
Strait Pharmaceutical Journal
基金
福建省中青年教师教育科技项目资助(JAT160796)
福建省中青年教师教育科技项目资助(JAT180162)
福建卫生职业技术学院“课程思政”试点建设项目(SZ2019013)
福建省卫生职业技术学院课题(2016-2-2)。
