HPLC测定余甘子茶中3种多酚成分及抗氧化活性研究

Determination of Three Polyphenol Components in Phyllanthus emblica L.Tea by High Performance Liquid Chromatography and Their Antioxidant Activity

福林酚法测定五个产地的余甘子茶中总多酚(TP)的含量,高效液相色谱法(High performance liquid chromatography,HPLC)分析没食子酸(GA)、柯里拉京(CO)、鞣花酸(EA)的含量,并对其抗氧化活性进行研究。结果表明:TP含量最高的是凉山的余甘子茶,其值为228 mg/g;漳州的余甘子茶中GA、CO、EA含量最高,分别为5.00、28.76、4.30 mg/g;在抗氧化活性研究中,昆明的余甘子茶提取液总还原能力较高,但凉山的余甘子茶提取液对DPPH自由基(DPPH·)和羟自由基(·OH)清除能力较好,EC50分别为7.46μg/mL和0.44 mg/mL,其对自发性肝脂质氧化抑制能力好于其它产地。相比较而言,凉山产地的余甘子茶作为开发天然抗氧化剂更具优势。

The phenolic contents of emblica(Phyllanthus emblica L.)fruit from five regions in China were measured by Folin phenol method in this study.Three main polyphenol components including ellagic acid(EA),gallic acid(GA)and corilagin(CO)in emblica were quantified by using high performance liquid chromatography(HPLC).Furthermore,the antioxidant activities of emblica ethanolic extracts were also evaluated.The results showed that the Liangshan sample exhibited a significantly higher phenolic content than other sampleswith the total polyphenol content of 228 mg gallic acid equivalents(GAE)/g.The contents of three compounds(GA,CO and EA)in Zhangzhou were 5.00,28.76 and 4.30 mg/g,respectively,which were markedly higher than other sample.The Kunming sample had the highest reducing power.The Liangshan sample was found to have the strongest antioxidant activities in inhibiting lipid peroxidation,scavenging DPPH radicals and hydroxyl radicals,with the EC50 vaule of 7.46μg/mL and 0.44 mg/mL.Thus,emblica fruit from Liangshan region might be a potential plant source of antioxidants.