臭氧水处理对冷藏期内草鱼鱼片挥发性成分的影响

Effect of Aqueous Ozone Treatment on Volatile Organic Compounds of Grass Carp Fillets during Refrigerated Storage

为研究臭氧水处理对草鱼鱼片挥发性成分的影响,本文采用快速气相电子鼻、顶空固相微萃取结合气相色谱-质谱联用(Gas chromatography-mass spectrometry,GC-MS)的方法对对照组、4 min处理组和8 min处理组在4℃冷藏0、5以及10 d时的样品进行检测分析。主成分分析(principal components analysis,PCA)与判别因子分析(discriminant factor analysis,DFA)结果表明臭氧水处理可有效改变草鱼鱼片的挥发性成分,贮藏期各样品气味差异明显;GC-MS检测发现整个贮藏期间样品包含43种主要挥发性物质,新鲜样品中主要含有己醛、戊醛、1-辛烯-3-醇、2,3-辛二酮等土腥气味物质,这些物质经臭氧水处理后可被有效去除,且去除效果随处理时间延长而增强;在贮藏期内,样品挥发性成分变化较大,但臭氧水处理组所含硫代乙酸甲酯、吲哚以及胺类等具有腐臭气味的挥发性物质含量均低于对照组。臭氧水处理可有效去除新鲜草鱼鱼片的土腥气味并具有延缓贮藏期内样品腐臭气味产生的作用。

The effects of aqueous ozone treatment on volatile organic compounds of grass carp fillets were systematically investigated.The volatile organic compounds(VOCs)changes of grass carp fillets with 0,5,and 10 d at 4℃ storage were assessed using E-nose and headspace solid-phase microextraction(HS-SPME)GC-MS analysis.Principal component analysis(PCA)and detection frequency analysis(DFA)showed differences in the VOCs between processing group and control sample during refrigerated storage.43 volatile components were identified by SPME/GC-MS.Fishy odor VOCs,such as hexanal,pentanal,1-octene-3-ol,2,3-octanedione and ethyl lactate,were detected in the fresh sample,while those VOCs reduced in the processing group with the extension of ozone treatment time.The composition of VOCs in all samples significantly changed during storage.The relative content of unpleasant VOCs,methyl thioacetate,indole,amines and so on,in treatment group was lower than that of the control sample.This study indicated that aqueous ozone treatment,possibly a promising strategy could reduce the unpleasant odor emission from fresh grass carp and delay the generation of off-flavor in the grass carp fillets during storage.