鱼腥草挥发油对多柔比星致大鼠心肌损伤的保护机制

Protective Mechanism of Essential Oil of Heartleaf Houttuynia Herb against Myocardial Injury Induced by Doxorubicin in Rats

目的:探讨鱼腥草挥发油对多柔比星(Doxorubicin,DOX)致大鼠心肌损伤的保护作用。方法:水蒸气蒸馏法制备鱼腥草挥发油,气相色谱质谱法(GC-MS)测定其成分,并评价其DPPH自由基清除作用、还原力、金属离子螯合能力。建立DOX心肌损伤模型,分为正常对照组、DOX模型组、鱼腥草挥发油25、50、100 mg/kg剂量组和维生素E(VE)500 mg/kg组;收集全血检测血常规;心肌组织HE染色;通过试剂盒检测血清中乳酸脱氢酶(LDH)活力;心肌组织过氧化氢酶(CAT)活力和丙二醛(MDA)含量;通过Agilent 7890B气相色谱仪测定盲肠内容物短链脂肪酸含量。结果:GC-MS数据显示鱼腥草挥发油主要含有28种化学成分。体外抗氧化实验表明鱼腥草挥发油具有较好的抗氧化能力;与DOX模型组相比,鱼腥草挥发油各组和VE组对DOX引起的体质量及血常规无显著变化,但能提高心肌组织中CAT活性,降低MDA含量及血清中LDH活性,且HE染色数据显示可抑制心肌组织的形态变化。同时,短链脂肪酸含量数据显示,DOX模型组含量显著下降,给予挥发油后无显著变化。结论:鱼腥草挥发油可通过抑制氧化应激,对DOX所致的心肌损伤发挥保护作用。

Objective:This work was designed to explore the protective effect of heartleaf houttuynia herb essential oil against doxorubicin(DOX)-induced myocardial injury in rats.Methods:In this work,heartleaf houttuynia herb essential oil was prepared by steam distillation,and its components were determined by gas chromatography-mass spectrometry(GC-MS).Subsequently,DPPH radical scavenging capacity,reducing power and chelating ability of metal ions were employed to evaluate its antioxidant activities.Furthermore,the myocardial injury model was established by intraperitoneal injection of DOX,which was divided into control group,DOX model group,25,50 and 100 mg/kg body dose of essential oil of heartleaf houttuynia herb groups and vitamin E(VE)500 mg/kg body group.Whole blood was collected for blood routine detection.Myocardial tissue was used for hematoxylin-eosin staining(HE)analysis.Moreover,malondialdehyde contents(MDA)in myocardial tissue,the activity of lactate dehydrogenase(LDH)in serum and catalase(CAT)in myocardial tissue were detected by the commercial kits.In addition,the content of short-chain fatty acids was determined by agilent 7890B gas chromatograph.Results:GC-MS data showed that the heartleaf houttuynia herb essential oil mainly contained 28 chemical components.Antioxidant activities in vitro tests further showed that the essential oil had potent antioxidant capacity.In three groups of heartleaf houttuynia herb essential oil and VE group,there were no significant changes in body weight and blood routine,when compared to DOX group.However,compared with the DOX model group,heartleaf houttuynia herb essential oil significantly increased CAT in myocardial tissue,while decreased MDA contents in myocardial tissue and LDH release in serum.HE dyeing analysis suggested that the essential oil could inhibit the morphological changes of myocardial tissue.At the same time,data showed that treatment with DOX alone remarkably decreased the short-chain fatty acid content,but there was no significant changes after administration of heartleaf houttuynia herb essential oil in rat.Conclusion:Heartleaf houttuynia herb essential oil possessed protective effects against DOX-induced myocardial injury by attenuating oxidative stress.