肝癌肝移植术后复发患者CD4^+T淋巴细胞ATP含量与亚群计数检测的临床意义

Detection of the ATP value and subgroup count of CD4^+T cell in patients with liver cancer recurrence after liver transplantation:its clinical significance

目的探讨肝癌肝移植术后复发患者CD4^+T淋巴细胞ATP含量与亚群计数检测的临床意义。方法选取2015年11月-2018年11月于上海长征医院因原发性肝癌行经典原位肝移植术的患者84例,根据肿瘤是否复发分为复发组(n=42)与未复发组(n=42)。在患者确诊复发当日及复发后第1、3、6个月采集外周血标本,采用ImmuKnowTM免疫细胞功能测定试剂盒检测CD4^+T淋巴细胞ATP含量,采用IOTest试剂盒检测其亚群计数。结果肝癌肝移植术后复发患者CD4^+T淋巴细胞ATP含量在复发当日以及复发后1、3、6个月均明显低于未复发患者(P<0.05)。与未复发患者比较,复发患者CD4^+T淋巴细胞亚群计数在复发后1个月内无明显变化,复发后3~6个月呈明显下降趋势(P<0.05)。结论肝癌肝移植术后复发患者免疫状态随时间延长逐渐降低,监测CD4^+T淋巴细胞ATP含量及亚群计数可较好地评估肝癌肝移植术后复发患者的免疫状态,在制定个体化方案上具有一定实用价值。

Objective To explore the clinical significance of detecting CD4^+T cell ATP and subgroup count in patients with liver cancer recurrence after liver transplantation.Methods From November 2015 to November 2018,84 patients with primary liver cancer underwent orthotopic liver transplantation in Shanghai Changzheng Hospital were selected and then divided into recurrence group(n=42)and non-recurrence group(n=42)according to whether the tumor recurred.Peripheral blood samples were collected on the day of diagnosis and 1,3,6 months after recurrence.ATP content in CD4^+T lymphocytes was detected using ImmuKnowTM immune cell function assay kit,subgroup counts were detected using IOTest.Results ATP content of CD4^+T lymphocytes in patients with liver cancer recurrence after liver transplantation was significantly lower than that in patients without recurrence at the day of diagnosis of recurrence,and 1,3,6 months after recurrence(P<0.05).The count of CD4^+T lymphocyte subsets in the reoccurence group had no significant change within 1 month after recurrence compared with the non-recurrence group.When the recurrence time continued to extend to 3 months and 6 months,there was a significant downward trend in both the two groups.Conclusion The immune status of patients with liver cancer recurrence after liver transplantation decreased gradually with the passage of time.Monitoring the ATP content and subgroup count of CD4^+T cells can better evaluate the immune status of patients with liver cancer recurrence after liver transplantation,being of certain practical value in formulating individualized programs.