大豆液泡加工酶VPE基因表达方式分析及其启动子预测

Analysis of Expression Patterns of VPE Gene in Soybean and Its Promoter Prediction

利用qRT-PCR方法,检测大豆液泡加工酶VPE基因在盐、干旱、低温、ABA条件下及大豆不同组织中的表达情况,结果显示VPE基因在盐、干旱、低温和ABA处理不同时间下,VPE基因的表达趋势基本相同,都是先随处理时间增加表达量升高,只是在盐处理中,最高表达量出现在5 h时,其它处理最高表达量出现在2 h,之后基因表达都下降。四种处理条件下,基因的表达变化不大,说明VPE基因基本不受盐、干旱、低温和ABA诱导。VPE基因在种子中的表达相对较高,尤其在90 DAF的种子中,相对于根的表达量为212.22倍。以大豆基因组DNA为模板,克隆VPE基因5’端上游启动子序列1914 bp,生物信息学预测表明VPE基因启动子中存在多种与种子特异表达相关的顺式调控元件,推测该启动子可能具有调控下游基因在种子中高表达的特性。

The qRT-PCR method was used to detect the expression of VPE gene in soybean under salt,drought,low temperature and ABA and different tissues of soybean.The results showed that the expression trends of VPE gene were basically the same under salt,drought,low temperature and ABA treatment at different times.The expression quantity increased firstly with the increase of processing time,than decreased.The highest expression occured at 5 hours under salt treatment,and the highest expression occured at 2 hours under drought,low temperature and ABA treatment.The expression of VPE gene changed little,which indicated that VPE gene was basically not induced by salt,drought,low temperature and ABA.The expression of VPE gene in seeds were relatively high,especially in 90 th DAF seeds,the relative expression of VPE gene to roots was 212.22 times.Using soybean genomic DNA as a template,1914 bp upstream promoter sequence of VPE gene at 5’end was cloned.Bioinformatics prediction showed that VPE gene promoter sequence contained a variety of cis-elements related to high expression in seeds,and speculated that VPE gene promoter might have seed-specific expression characteristics.